User Manual

9
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3.4 Gel Casting using External Gel Caster
1.Levelexternalgelcasterusingwhite
thumbscrewsonbase.Placegeltray
intoExternalGelCaster.Endsofgel
trayfrictiontontoplace.Positionthe
combintoreceptacleslotsofgeltray.
Addadditionalcombifdesired.
2.Makeagarosesolutionaccordingto
manufacturer’sinstructions.Stains may
be added during this step. Use accord-
ing to manufacturer’s recommenda-
tions. Use caution when handling hot
agarose solution! Pourmoltenagarose
solutionintogeltray.Allowtocool
uncovereduntilagaroseturnopaque.
NOTE:(Optionalstorage)Pre-castgels
canbestoredforuptoonemonthif
wrappedinplasticwrapandstoredat
4ºC.Removecombspriortostoring.
3.Removecombandpositionthetrayin
theunit,remembering,currenttravels
fromnegative(blackcathode)towards
positive(redanode).
Go to Section 3.5
6.Removecomb(s).
7.Removebafesfromtheircastingposi-
tion.
8.Turnbafes180º(handlesfacingin)
andplaceintothebafestorageslots
providedateitherendoftheunitnear
bothterminals.
Go to Section 3.5