User Manual

8
Midi-Horizontal Instructions 9-24-13
3.3 Gel Casting in Unit using tapered bafes
1. Removelidbypushingdownonwhite
thumbpostswhilepullinguplidwith
ngers.DO NOT PULL ON POWER
CORDS!
2. Placegeltrayincenterofhorizontalgel
bed.
3. Removetaperedbafesfromstorage
positionandslidethemsnuglyintothe
bafeguidesnexttothebed,handles
to the outside.Thetaperedbafeswill
restfrictiontightagainstthegelbed.Do
NOTpressthebafestootightlyintothe
stops.
4. Positionthegelcomb,remembering,
currenttravelsfromnegative(blackcath-
ode)towardspositive(redanode).Add
additionalcombsifdesired.
5.Makeagarosesolutionaccordingto
manufacturer’sinstructions.Use caution
when handling hot agarose solu-
tion! Pourmoltenagarosesolutioninto
geltray.Allowtocooluncovereduntil
agaroseturnopaque.Stains may be
added during this step. Use according to
manufacturer’s recommendations.