User Manual

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SECTION 1

General Information

1.1 Introduction

TheHorizontalMidi-Gelsystems,(GC)MGU-402T,(GC)MGU-502T,and(GC)GCMGU-

602T,provideacompactformattorapidlyseparateandidentifyDNAandRNAfrag-

ments,PCRproductsandsyntheticoligonucleotides,whileusingminimalquantitiesof

expensivereagents.Thetaperedbafesystemallowsgelcastingdirectlyontotheunit

orremovabletrayswithouthavingtosealwithagaroseplugsortape.Thegelbedand

geltrayareUVtransparent.Combsareofferedinseveralthicknessesandformatsto

accommodateawidevarietyofexperimentalapplications.

1.2 Specications

 Constructions

  Bufferchamber,lid Acrylic

  Electrodes  Platinumwire.012”diameter

  Powercords  FRSiliconerated7500VDC,200mA,65ºC 

  Taperedbafes  Polycarbonate

  Combs   Polycarbonate,AnodizedAluminumorTeon

  Combbacking  Acrylic 

  UVTGelTray(optional) U-Vtransparentacrylic

  SafetyCertication EN61010-1-1993(IEC1010-1)

*Calculated using gel thickness of 0.5cm

1.3 Safety

PowertotheMidi-HorizontalunitsistobesuppliedbyanexternalDCvoltagepower

supplythatmustbegroundisolatedsothattheDCvoltageoutputoatswithrespectto

ground.Foranypowersupplyused,themaximumspeciedoperatingparametersfor

theunitsare:

Maximum Limits

   250VDCvoltage

   15wattspower

   60mAcurrent

   50*Cambienttemperature

Currenttotheunit,providedfromtheexternalpowersupply,mustentertheunitthrough

thelidassembly,providingasafetyinterlocktotheuser.Currenttotheunitisbroken

whenthelidisremoved.Donotattempttousetheunitwithoutthesafetylid.Always

turnthepowersupplyoffbeforeremovingthelid,orwhenworkingwiththeunitinany

way.Followsafetyprecautionsspeciedbythepowersupplymanufacturer.

Model # (GC)MGU-402T (GC)MGU-502T (GC)MGU-602T

Shippingweight 4lb. 6lb. 7lb.

OverallSizeWxLxH(cm) 13x22.5x14 16.7x21.5x14 16.7x27.5x14

GelBedSizeWxL(cm) 10.5x11 14x10 14x16

RecommendedBufferVolume 550ml 700ml 750ml

AgaroseSolutionRequired* 50ml 60ml 100ml

#ofSlotsinGelTray 2 2 4

Pathlength/Slot(cm) 4.0 3.5 3.0

Voltagelimit 250VDC 250VDC 250VDC