User Manual

10
Midi-Horizontal Instructions 9-24-13
3.5 Running the Gel
1. Addenoughrunningbuffertollboth
reservoirsandoverowthesurfaceofthe
geltoadepthof2-3mm.Flushoutanyair
bubblesinthewells.Stains may be added
during this step. Use according to manu-
facturer’s recommendations.
2.Loadthesamplesintosamplewells.Do
notforgettoloadDNAsizestandardlad-
ders.Forbestvisibilityloadsampleson
contrastingbackgroundcolorsuchasred
orblack.
Safe stains may be added during this step.
Use according to manufacturer’s recom-
mendations.
3.Placethesafetycoverontheunit.Con-
nectpowerleadstopowersupply,match-
ingthecolor-codedredtoredandblackto
black.See Section 4.1 for recommended
power conditions.Beginseparationby
electrophoresis.
3.6 Removing the Gel
1. Turnpowersupplyoffanddisconnect
theleadsfromthepowersupply.Remove
thesafetycoverfromtheMidi-Gelunitby
placingthumbsonwhitepostsandpushing
downwhilepullingupwithngersunderlid.
DO NOT PULL ON POWER CORDS!
2.Gentlyliftthegeltrayfromtheunit.Al-
ways wear gloves, eye protection and
protective clothing if buffer and /or gel
contain Ethidium Bromide.EthidiumBro-
mideisapowerfulmutatgen;gloves,eye
protectionandprotectiveclothingshould
alwaysbewornwhenhandlingthegelor
buffersolutions.Viewseparatedfragments
underUVlight,usingproperprotection
foreyesandskin(seemanufacturer’s
instructions).Alternatively, Safe Stains may
be used and viewed using blue light or UV
excitation.