Manual

7
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SECTION 3
Instructions for Use
3.1 Mini-Gel Unit Preparation and Gel Casting
1.PlaceMini-Gelunitonalevelworksurface
inanauthorizedworkarea.Placetheadjust-
ableheightcombassemblyoverthegelbed
orgeltray.Loosenthewhitescrewsholding
thecombtothebackingandadjustcomb
depth(generally1mm).Gentlytightenthe
adjustingscrewsandsetthecombaside.
SeeTable1forcombvolumes.
2.Powerleadsareshipped
separatelyandmust be at-
tached before using your
Midi-Gel. Threadthepower
leadendsintothelidrecep-
taclesandrotateinclockwise
directiontohandtighten.
Safetycover
3.2 Stain Options
1) Safe Stains
SafeStainsarealessmutatgenicalternativetoDNAandRNAvisualizationthan
EthidiumBromideanddonotrequireexposuretoUVlight.FollowtheSafeStain
manufacturer’sinstructionsandrecommendations.Safestainscanbeaddedat4
differentstagesofelectrophoresis:
 a)intotheagarosegelsolution-Sections3.2.5and3.3.2
 b)intorunningbuffer-Section3.4.1
 c)intosampleloadingbuffer-Section3.4.2
 d)afterelectrophoresis-Section3.5.2
2) Ethidium Bromide (EtBr) Staining
EtBrisaMutagen.ALWAYSweargloves.Disposeofsolutionsinaccordancewith
thesafetyregulationsofyourinstitution.EtBrcanbeaddedat3differentstagesof
electrophoresis:
 a)intotheagarosegelsolutionafterthesolutionhasbeenmademoltenand
 thetemperaturehasdroppedto55°C-Sections3.2.5and3.3.2
 b)intorunningbuffer-Section3.4.1
 c)aftertheelectrophoresisruninastainsolution.Useatanalconcentration
 of0.5-1µg/ml.-Section3.5.2
Powerleads