Mini-Horizontal Systems MGU-102T MGU-202T GCMGU-102T GCMGU-202T INSTRUCTION MANUAL
TABLE OF CONTENTS Important User Information 3-4 Section 1 1.1 1.2 1.3 General Information Introduction 5 Specifications 5 Safety 5 Section 2 2.1 2.2 Description of parts Unpacking Components/Assembly Section 3 3.1 3.2 3.3 3.4 3.5 3.6 3.
ENGLISH IMPORTANT USER INFORMATION This Instruction Manual will explain how to use this product safely and effectively. Please read and carefully follow the instruction manual in its entirety. The triangle/exclamation mark symbol alerts the user of the product to important operational, maintenance, and/or warranty requirements. The triangle/lighting bolt symbol alerts the user of the product to potentially hazardous electrical exposure.
FRANÇAIS INFORMATION IMPORTANTE À L’USAGE DES UTILISATEURS ESPAÑOL Le présent manuel d’utilisation explique la manière de se servir efficacement du produit en conditions de sécurité. Il est recommandé de soigneusement lire la totalité du manuel, avec ses consignes et ses instructions. El presente instructivo explica la manera de usar este producto en forma segura y efectiva. Sírvase leerlo en su totalidad y seguir detenidamente las indicaciones que contiene.
SECTION 1 General Information 1.1 Introduction Horizontal Mini-Gel systems (GC)MGU-102T and (GC)MGU-202T provide a compact format to rapidly separate and identify DNA and RNA fragments, PCR products and synthetic oligonucleotides, while using minimal quantities of expensive reagents. The tapered baffle system allows gel casting directly onto the unit or removable trays without having to seal with agarose plugs or tape. The gel bed and gel tray are UV transparent.
SECTION 2 Description of Parts 2.
SECTION 3 Instructions for Use 3.1 Mini-Gel Unit Preparation and Gel Casting 1. Place Mini-Gel unit on a level work surface in an authorized work area. Place the adjustable height comb assembly over the gel bed or gel tray. Loosen the white screws holding the comb to the backing and adjust comb depth (generally 1mm). Gently tighten the adjusting screws and set the comb aside. See Table 1 for comb volumes. 2. Power leads are shipped separately and must be attached before using your Midi-Gel.
3.2 Gel Casting in Unit using tapered baffles 1. Lift lid so that it rests in up position. 2.Place gel tray in center of horizontal gel bed. 3. Remove black tapered baffles from storage position and Slide them snugly into the grooves next to the bed, handles to the outside. The tapered baffles will rest friction tight against the gel bed. Do NOT press the baffles too tightly into the stops. 4.
6. Remove comb. 7. Remove black baffles from their casting position. 8.Turn baffles 180º (handles facing in) and place into the baffle storage slots provided at either end of the unit near both terminals. Go to Section 3.4 3.3 Gel Casting using external gel caster 1. Level external gel caster using white thumb screws on base. Place Gel tray into external gel caster. Ends of gel tray friction fit onto place. Position the comb into receptacle slot in gel tray. Add additional comb if desired. 2.
3.4 Running the Gel 1. Add enough running buffer to fill both reservoirs and overflow the surface of the gel to a depth of 2-3mm. Flush out any air bubbles in the wells. Stains may be added during this step. Use according to manufacturer’s recommendations. 2. Load the samples into sample wells. Do not forget to load DNA size standard ladders. For best visibility load samples on contrasting background color such as red or black. Safe stains may be added during this step.
3.6 Comb Specifications (GC)MGU-102T STANDARD COMB OPTIONS for 5.5cm wide for (GC)MGU-102T, adjustable height material: Polycarbonate or PTFE-coated aluminum* tooth depth: 6.35mm overall length: 5.0cm Cat. # MGC-1001 MGC-1003E MGC-1003M MGC-1008 MGC-1010 MGC-1012 MGC-1014 MGC-1016 MGC-2001 MGC-2003E MGC-2003M MGC-2008 MGC-2010 MGC-2012 MGC-2014 MGC-2016 # of Teeth Thickness of Teeth (mm) Width of Teeth (mm) Recommended Max. Sample Vol.
3.6 Comb Specifications, con’t. (GC)MGU-202T STANDARD COMB OPTIONS for 7.5cm wide for (GC)MGU-202T, adjustable height material: Polycarbonate or PTFE-coated aluminum* tooth depth: 6.35mm overall length: 7.0cm Cat. # MHC-1001 MHC-1003E MHC-1003M MHC-1005 MHC-1008 MHC-1010 MHC-1012 MHC-1014 MHC-1016 MHC-2001 MHC-2003E MHC-2003M MHC-2005 MHC-2008 MHC-2010 MHC-2012 MHC-2014 MHC-2016 # of Teeth Thickness of Teeth (mm) Width of Teeth (mm) Recommended Max. Sample Vol.
SECTION 4 Running Conditions 4.1 Recommended Power The recommended power conditions for optimal resolution are 50 to 150V, constant, and 25 to 80 mA. The usual run time will vary for the voltage chosen but should range from 15 to 60 minutes, monitoring nucleic acid migration by progress of marker dyes. Constant power is not a necessity, but it produces uniform heat throughout the run. Be sure the polarity is correct i.e.
SECTION 5 Maintenance of Equipment 5.1 Care and Handling The plastic components of the Mini-Horizontal units are fabricated from acrylic and polycarbonate. Electrodes and connectors are made from pure platinum, stainless steel, and chrome plated brass. As with any laboratory instrument, adequate care ensures consistent and reliable performance. After each use, rinse buffer chamber, gel tray and combs with de-ionized water. Wipe dry with a soft cloth or paper towel, or allow to air dry.
SECTION 6 Ordering Information CAT. # DESCRIPTION MINI-HORIZONTAL SYSTEMS Color- Coded Option: To specify color add the following color code to the end of the catalog #: -Y (Yellow), -P (Purple), -B (Blue), -PK (Pink), or -G (Green). Note: Mini-Horizontals are clear unless otherwise specified. MGU-102T MGU-202T GCMGU-102T GCMGU-202T Standard Mini-Horizontal Kit, CE, gel dimension 5.5cm(w) x 8.5cm(l) Standard Mini-Horizontal Kit, CE, gel dimension 7.
CONTACT INFORMATION Online Ordering www.cbsscientific.com Telephone Local and International: 858-755-4959 Toll Free: 800-243-4959 Sales E-mail Address sales@cbssci.com Technical Service E-mail Address technicalservice@cbssci.com Mailing Address P.O.
