Owner manual

CASSETTE ASSEMBLY

9.Removeentireassemblyfrompyrexdishandloadintodesiredposition(slot)inbufferchamberwithblack

screenfacingtowardsblackterminal(cathode)andredscreenfacingtowardsredterminal(anode).

3.3 STANDARD ELECTRO-BLOT TRANSFER

1. Fillthechamberwithtransferbuffer.(Buffersshouldbepreparedfreshwithreagentgradechemicalsand

pre-cooled).Thetransferbuffershouldcomeuptothetopoftheplatinumlabyrinth.Thebuffershouldnot

comeincontactwiththebananaplugswhenthegelcassettesandwich(es)areimmersedintheunit.

2. Align safety cover over the unit and carefully attach. Begin cycling of coolant.

3. Connecttheleadstothepowersupply,matchingthecolor-codedredtoredandblacktoblack.SeeSection

4.1forrecommendedpowerconditions.

4. Transfertimeswillvaryaccordingtoseveralparameters.Optimizationofelectro-blottingtransfersmustbe

determinedempirically.Keepinmindthefollowingprinciplesthatgovernthemovementofmoleculesofgel

electrophoresis:

• Thickerorhigherpercentagegelswilltakelongertotransferthanthinnerorlowerpercentagegels.

• Largemoleculeswillneedextendedtransfertimestocompletelytransfer.

• Actualtransfertimesfordenedconditionscanbeapproximatedbyrunningmolecularweightstandards.

3.4 REMOVING THE CASSETTES

1. Turnthepowersupplyoffanddisconnecttheleadsfromthepowersupply.

2. Removethesafetycoverfromtheunit,byplacingthumbsonwhitepostsnexttored&blackconnectors,

thenpushingdownwhilepullingupwithngersunderlid.DONOTpullonpowercords.

3. Gentlyliftthecassettefromtheunit.Alwaysweargloves,eyeprotectionandprotectiveclothingifbufferand/

orgelcontainEthidiumBromide.EthidiumBromideisapowerfulmutagen;gloves,eyeprotectionandpro-

tective clothing should always be worn when handling the gel or buffer solutions. See Material Data Safety

Sheets.

4. Marktheorientationofthemembranewithapencilorbycuttingoffacornerandtakeapartthecassette

carefully.

5. Processmembraneaccordingtotypeoftransferandmanufacturer’srecommendations.