Owner manual

EBU-222 Instruction Manual, version 7/28/2011

www.cbsscientic.com

SECTION 1

General Information

1.1 Introduction

Electroblottingisafaster,easier,andmoreefcientmethodtopermanentlytransferPAGEgelstonitrocellulose

andnucleicacidstonylon,thancapillary(passivediffusion)blotting.Forasimilartransfer,traditional“capillary

blotting”cantake6-24hourstocompletecomparedtoonly2hoursorlessusingelectroblotting.TheC.B.S.Sci-

enticElectrophoreticBlottingSystemscomeequippedwith;twoelectrodepanels,onecolor-codedgelcassette

assembly,electrophoresistankwithcoolingchamberandsafetycoverwithattachedpowerleads.TheEBU-222

gelcassettesizeis20cmx21.5cmandcantransferuptothreegelsatonetime.TheEBU-222gelcassettesize

is9x11cmandcantransferupto2gelsatonetime.

1.2 Specications

Constructions:

  Bufferchamber,safetycover   Acrylic

  Electrodepanels    Acrylicandplatinumwire.010”diameter

  Powercords     FRPolypropylene/Silicone,rated7500V,200mA,65° C

Gel cassette Perforated acrylic panels red and black, two

sponge pads

  SafetyCertication    EN61010-1-1993(IEC1010-1)

Shipping Weight 5 lbs.

  OverallSize(l)x(w)x(h)cm   19x11x28.5

  MaximumGelSize-cm   18x18

  Distancebetweenelectrodes-cm  8.7

  Recommendedbuffervolume

1 cassette 3.5 liters

2 cassettes 3.0 liters

1.3 Safety

PowertotheElectro-BlotteristobesuppliedbyanexternalDCvoltagepowersupplythatmustbeground

isolatedsothattheDCvoltageoutputoatswithrespecttoground.Foranypowersupplyused,themaximum

speciedoperatingparametersfortheunitsare:

     MaximumLimits

100 VDC

70 watts power

     1500mAcurrent

     50ºCambienttemperature

Currenttotheunit,providedfromtheexternalpowersupply,mustentertheunitthroughthelidassembly,provid-

ingasafetyinterlocktotheuser.Currenttotheunitisbrokenwhenthelidisremoved.Donotattempttousethe

unitwithoutthesafetylid,andalwaysturnthepowersupplyoffbeforeremovingthelid,orwhenworkingwiththe

unitinanyway.Followsafetyprecautionsspeciedbythepowersupplymanufacturer.