Manual

50
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3.14 Instructions for Use Buffer Siphon Pump
Dispose of solutions in accordance with the safety regulations of your institution.
Fig. 3-12
3.15 Maximum Well/Comb Volumes
NOTE: To calculate sample well volume expressed in millimeters (mm) of height, divide maximum volume by
tooth depth.
1. Attach pump assembly to corner of tank as shown at left.
(Fig 3-12)
2. Place the discharge tubing into a waste carboy located
LOWER than the pump inlet.
3. Begin siphon with half strokes until liquid starts to ow.
Leave shaft, fully extended to allow siphon to operate.
4. To stop siphon, depress handle and remove pump from
tank.
VGC xxxx COMB OPTIONS DEEPER WELLS
overall length = 13.1cm
Cat. # tooth depth
(mm)
# of
teeth
thickness
of teeth
width of
teeth
maximum
well volume
(µl)
VGC-0716 19.1 16 0.75 5.2 74
VGC-0720 17.7 20 0.75 4.2 56
VGC-0722 17.7 22 0.75 4.35 33
VGC-0729 17.7 29 0.75 2.9 38
VGC-0730 17.7 30 0.75 2.8 37
VGC-1016 19.1 16 1.0 5.2 98
VGC-1020 19.1 20 1.0 4.2 74
VGC-1022 17.7 22 1.0 4.35 76
VGC-1029 17.7 29 1.0 2.9 51
VGC-1030 17.7 30 1.0 2.8 49
VGC-1516 19.1 16 1.5 5.2 147
VGC-1520 17.7 20 1.5 4.2 111
VGC-1522 17.7 22 1.5 4.35 114
VGC-1529 17.7 29 1.5 2.9 77
VGC-1530 17.7 30 1.5 2.8 74
VGC xxxx -177 COMB OPTIONS SHORT WELLS
overall length = 13.1cm
Cat. # tooth depth
(mm)
# of
teeth
thickness
of teeth
width of
teeth
maximum
well volume
(µl)
VGC-0716-177 10 16 0.75 5.2 39
VGC-0720-177 10 20 0.75 4.2 31
VGC-0722-177 10 22 0.75 4.35 33
VGC-0729-177 10 29 0.75 2.9 22
VGC-0730-177 10 30 0.75 2.8 21
VGC-1016-177 10 16 1.0 5.2 52
VGC-1020-177 10 20 1.0 4.2 42
VGC-1022-177 10 22 1.0 4.35 43
VGC-1029-177 10 29 1.0 2.9 29
VGC-1030-177 10 30 1.0 2.8 28
VGC-1516-177 10 16 1.5 5.2 78
VGC-1520-177 10 20 1.5 4.2 63
VGC-1522-177 10 22 1.5 4.35 65
VGC-1529-177 10 29 1.5 2.9 44
VGC-1530-177 10 30 1.5 2.8 42