Manual

Cipher Genetic Analysis System
DTSK Instruction Manual, version 8/30/2011
39
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3.6GelCastingTechniques-continued
C. Vertical gradient gel casting using gradient maker (GM-40) and a mini-pump for DGGE.
1. Alternatively, you may choose to use a “mini-pump” or other peristaltic pump to cast gels as
shown in (Fig 3-5). If so, secure the gradient maker to a ring stand and connect the outlet
tubing to the mini-pump tubing adapter. Connect tubing from mini-pump to a 20ga. needle for
afxing between glass plates.
Fig. 3-5
2. For TTGE and SSCP single percentage gels, replace the gradient maker with ask (more
consistent than syringe).
  Refertosection3.7forGradientGelFormulaProtocol.
See section 4.3 for running conditions.