Manual

2
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TABLE OF CONTENTS
Important User Information 3-4
Section 1 General Information
1.1 Introduction 5
1.2 Specications  6
1.3 Safety 6
Section 2 Description of parts
2.1 Unpacking 7
2.2 Components/Assembly 7
Section 3 Instructions for Use
3.1 Unit Set-up and Unpacking 8-9
3.2 Heater/Stirrer General Set-Up 10-24
3.3 Heater/Stirrer Programming 25-34
3.3.1 Setting Operational Parameters and Functions 25
3.3.2Entering/ModifyingaTemperatureProgram 26-30
3.3.3 Running a Temperature Program 31-32
3.3.4 Setting Preferences 32
3.3.5 Heater/Stirrer Error Messages 33-34
3.4 Running the Heater/Stirrer 35
3.5 Preparation/Cleaning of Glass Plates 35
3.6 GelCastingTechniques 36-37
3.7 Vertical Gradient Formation 38-42
3.8 Preparation of the Cassettes 43
3.9 Buffer Cycling Connections 44
3.10 Running the Gels 45
3.11 RemovingtheGels 46
3.12 Perpendicular Gel Casting 47
3.13 Perpendicular Gradient Formation 48-49
3.14 Buffer Siphon Pump Instructions for use 50
3.15 Maximum Well/Comb Volumes 50
Section 4 Running Conditions 51
4.1 Recommended Power 51
4.2 Recommended Buffers 51
4.3 Recommended Running Conditions 52
4.4 SSCP Running Conditions 52
4.5 References 53
Section5 MaintenanceofEquipment
5.1 Care and Handling 54
5.2 Maintenance & Calibration 55
Section6 Heater/StirrerTroubleshooting 56-57
Section 7 Accessories 58