Manual
2
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TABLE OF CONTENTS
Important User Information 3-4
Section 1 General Information
1.1 Introduction 5
1.2 Specications 6
1.3 Safety 6
Section 2 Description of parts
2.1 Unpacking 7
2.2 Components/Assembly 7
Section 3 Instructions for Use
3.1 Unit Set-up and Unpacking 8-9
3.2 Heater/Stirrer General Set-Up 10-24
3.3 Heater/Stirrer Programming 25-34
3.3.1 Setting Operational Parameters and Functions 25
3.3.2Entering/ModifyingaTemperatureProgram 26-30
3.3.3 Running a Temperature Program 31-32
3.3.4 Setting Preferences 32
3.3.5 Heater/Stirrer Error Messages 33-34
3.4 Running the Heater/Stirrer 35
3.5 Preparation/Cleaning of Glass Plates 35
3.6 GelCastingTechniques 36-37
3.7 Vertical Gradient Formation 38-42
3.8 Preparation of the Cassettes 43
3.9 Buffer Cycling Connections 44
3.10 Running the Gels 45
3.11 RemovingtheGels 46
3.12 Perpendicular Gel Casting 47
3.13 Perpendicular Gradient Formation 48-49
3.14 Buffer Siphon Pump Instructions for use 50
3.15 Maximum Well/Comb Volumes 50
Section 4 Running Conditions 51
4.1 Recommended Power 51
4.2 Recommended Buffers 51
4.3 Recommended Running Conditions 52
4.4 SSCP Running Conditions 52
4.5 References 53
Section5 MaintenanceofEquipment
5.1 Care and Handling 54
5.2 Maintenance & Calibration 55
Section6 Heater/StirrerTroubleshooting 56-57
Section 7 Accessories 58