Instruction Manual
C. B. S. Scientific 5 Dual Slab Gels
SECTION 1
General Information
1.1 Introduction
C.B.S. Scientific offers the Dual Slab Gel Systems designed to meet a wide variety of
applications. These systems are designed for separating and characterizing protein and can be
adapted for short sequencing runs with the appropriate accessories. Two gels can be run
simultaneously under identical buffer conditions. The cooling chamber assures constant
temperature during electrophoresis. Gel casting can be accomplished using our convenient and
unique Gel Wrap™, or by using our multiple gel casting chambers (see Section 6, Dual Slab Gel
Kits and Accessories).
With the use of special accessories, two dimensional (2-D) gel electrophoresis can be performed
the 220mm vertical system (cat. # DSG-200-02). First dimension I.E.F. tube gels are run on the
unit using IEF Tube Gel Adapters (described in the catalogue). Buffer surrounds the tubes and
is cooled by the internal cooling chamber for temperature-controlled runs. Second dimension
separation is performed by using the standard accessories. A complete description of 2-D
protocol is included when ordering the I.E.F. Tube Gel Adapters (cat. # TGA-200K).
Other applications include; mobility shift assays, antibody super shift assays, Rnase protection
assays, DNA footprinting, differential display, DNA paternity testing and forensic analysis.
1.2 Specifications
Constructions:
Buffer chamber, safety cover Acrylic
Electrodes Platinum wire .012" diameter
Power cords FR Urethane rated 7500VDC,
200mA, 65°C
Combs Teflon
Glass plates Soda-lime float glass
Spacers PVC
Clamps Polypropylene, stainless steel
Safety Certification EN61010-1-1993 (IEC1010-1)
Table 2
Specifications
Dual Slab Gel Unit:
Shipping
weight
Overall unit
size cm
(l) x (w) x (h)
Distance between
electrodes mm
Recommended
buffer volume
mls
Voltage
Limit
VDC
145mm
12 lb. 24 x 23 x 21.91 250 300 upper
125-200 lower
725
160mm
12 lb. 24 x 23 x 23.33 265 300 upper
125-200 lower
800
170mm
12 lb. 24 x 23 x 24.29 275 300 upper
125-200 lower
850
190mm
12 lb. 24 x 23 x 26.35 295 300 upper
125-200 lower
950
220mm
12 lb. 24 x 23 x 29.12 325 300 upper
125-200 lower
1100
280mm
13 lb. 24 x 23 x 35.24 385 300 upper
125-200 lower
1400
387mm
16 lb. 24 x 23 x 45.94 480 300 upper
125-200 lower
1900