INSTRUCTION MANUAL Dual Vertical Slab Gel Systems DSG-125-02 DSG-160-02 DSG-170-02 DSG-190-02 DSG-200-02 DSG-250-02 DSG-400-02
TABLE OF CONTENTS Page Important User Information 3-4 Section 1 1.1 1.2 1.3 General Information Introduction Specifications Safety 5 5 6 Section 2 2.1 2.2 Description of parts Unpacking Components/Assembly 7 7 Section 3 3.1 3.2 3.3 3.4 3.5 3.6 Instructions for Use Dual Slab Gel Unit Preparation Preparation/Cleaning of glass plates Gel Casting Running the Gel Removing the Gel Maximum Well/Comb volumes 8 8 8-10 10-11 11 11 Section 4 4.1 4.2 4.
ENGLISH IMPORTANT USER INFORMATION This Instruction Manual will explain how to use this product safely and effectively. Please read and carefully follow the instruction manual in its entirety. The triangle/exclamation mark symbol alerts the user of the product to important operational, maintenance, and/or warranty requirements. The triangle/lighting bolt symbol alerts the user of the product to potentially hazardous electrical exposure.
FRANÇAIS INFORMATION IMPORTANTE À L'USAGE DES UTILISATEURS ESPAÑOL Le présent manuel d'utilisation explique la manière de se servir efficacement du produit en conditions de sécurité. Il est recommandé de soigneusement lire la totalité du manuel, avec ses consignes et ses instructions. El presente instructivo explica la manera de usar este producto en forma segura y efectiva. Sírvase leerlo en su totalidad y seguir detenidamente las indicaciones que contiene.
SECTION 1 General Information 1.1 Introduction C.B.S. Scientific offers the Dual Slab Gel Systems designed to meet a wide variety of applications. These systems are designed for separating and characterizing protein and can be adapted for short sequencing runs with the appropriate accessories. Two gels can be run simultaneously under identical buffer conditions. The cooling chamber assures constant temperature during electrophoresis.
1.3 Safety Power to the Dual Slab Gel units is to be supplied by an external DC voltage power supply that must be ground isolated so that the DC voltage output floats with respect to ground.
Description of Parts 2.1 Unpacking Please verify that your unit comes complete with the following components: Dual Slab Gel Kit: • • • • • • • • • • 2.
SECTION 3 Instructions for Use 3.1 Dual Slab Gel Unit Preparation 1. Place Dual Slab Gel unit on a level work surface in an authorized work area. Level the unit by adjusting the four white nylon thumbscrew until the bubble is centered in the level. Be sure that all four of the screws are touching the bench. 2. Verify that the comb, spacer set and Gel Wrap™ gasket are the same thickness, by assembling without gel casting. 3.
4. Place the notched plate on top of the bottom assembly, starting from the bottom edge and gently easing the plate down. Verify the gasket is smooth around the edges and then clamp along the bottom. 5. Lift the assembly and stand it on the base of the clamps. For leveling, push glass plate assembly down until it stops against clamp body. Clamp the sides of the assembly with additional casting clamps on either side. As each clamp is attached, be sure the gasket is aligned between the plates forming a seal.
3.4 Running the Gel 1. Attach plate set(s) to unit with smaller or notched plate facing towards the upper buffer reservoir. If using a precast gel stored at 4°C, allow to warm to room temperature. Use white clamps with mouth open (cat. # GPC0001) to attach plate set to unit, clamping each side to the sandwich. 2. Pour freshly prepared buffer in upper and lower chamber. Using a pipette or syringe, thoroughly flush out the wells in the glass plate sandwich with buffer from the upper chamber.
3.5 Removing the Gel 1. Turn the power supply off and disconnect the leads from the power supply. Remove the safety cover from the unit, by placing thumbs on white posts next to red & black connectors, then pushing down while pulling up with fingers under lid. DO NOT pull on power cords. 2. Drain upper reservoir using a pipette or syringe. Detach side clamps and remove gel sandwich. Carefully separate, making sure that the gel remains attached to one plate.
SECTION 4 Running Conditions 4.1 Recommended Power Precise electrophoresis conditions will vary according to the number and type of gels used, buffer conditions employed, power input, and the general goal of the experiment. Refer to the reference section for in depth discussions on practical and theoretical approaches to protein gel electrophoresis. Using standard SDS-PAGE buffer systems apply 1-10 VDC/cm of gel. For sequencing applications use 50 VDC/cm.
4.2 Recommended Buffers and Reagents-continued BUFFERS: Agarose TBE (1X solution): 0.089M Tris base 0.89M Boric acid 0.002M EDTA pH 8.3 Protein Denaturing TG-SDS(1X): 0.025M Tris base 0.192M Glycine 0.1%(w/v) SDS pH3 DNA Sequencing TTE (Tris/Taurine/EDTA)(1X): 1.78M Tris 0.57M Taurine 0.01M EDTA Na2-2H20 4.3 Page/Agarose Slab TAE (1X): 0.04M Tris-acetate 0.001M EDTA pH 8.0 Denaturing/Non-Denaturing/ Nylon Blotting TT (Tris-Taurine( (1X): TT-SDS (1X): 0.1M Tris base 0.1M Tris base 0.1M Tricine 0.
SECTION 5 Maintenance of Equipment 5.1 Care and Handling The plastic components of the Dual Slab Gel units are fabricated from acrylic and polycarbonate. Electrodes and connectors are made from pure platinum, stainless steel, and chrome plated brass. As with any laboratory instrument, adequate care ensures consistent and reliable performance. After each use, rinse buffer chamber, glass plates, spacers and combs with de-ionized water. Wipe dry with a soft cloth or paper towel, or allow to air dry.
SECTION 6 Dual Slab Gel Kits and Accessories Cat. # I Item DSG-125 or DSG-125-02 Dual Slab Gel Kit, 145mm height. Includes 2 combs, 2 Gel Wrap spacer sets, 2 Gel Wrap™ Glass plate sets, 2 each Gel Wrap™ Gaskets, and 12 clamps. DSG-160 or DSG-160-02 Dual Slab Gel Kit, 160mm height. Includes 2 combs, 2 Gel Wrap spacer sets, 2 Gel Wrap™ Glass plate sets, 2 each Gel Wrap™ Gaskets, and 12 clamps. DSG-170 or DSG-170-02 Dual Slab Gel Kit, 170mm height.
SPACERS for GEL WRAP (PVC) Cat. # Spacer dimensions GEL WRAP™ GASKET Cat. # Gasket thickness x plate size VGS-7512R 0.75mm x 14.5cm VGS-7516R 0.75mm x 16cm VGS-7517R 0.75mm x 17cm VGS-7519R 0.75mm x 19cm VGS-7520R 0.75mm x 22cm VGS-7525R 0.75mm x 28cm VGS-7540R 0.75mm x 40cm VGS-1012R 1.0mm x 14.5cm VGS-1016R 1.0mm x 16cm VGS-1017R 1.0mm x 17cm VGS-1019R 1.0mm x 19cm VGS-1020R 1.0mm x 22cm VGS-1040R 1.0mm x 40cm VGS-1512R 1.5mm x 14.5cm VGS-1516R 1.5mm x 16cm VGS-1517R 1.5mm x 17cm VGS-1519R 1.
VGS-7512 VGS-7516 VGS-7517 VGS-7519 VGS-7520 VGS-7525 VGS-7540 VGS-1012 VGS-1016 VGS-1017 VGS-1019 VGS-1020 VGS-1025 VGS-1040 VGS-3012 VGS-3016 VGS-3017 VGS-3019 VGS-3020 VGS-3025 VGS-3040 0.75mm x 14.5cm 0.75mm x 16cm 0.75mm x 17cm 0.75mm x 19cm 0.75mm x 22cm 0.75mm x 28cm 0.75mm x 40cm 1.0mm x 14.5cm 1.0mm x 16cm 1.0mm x 17cm 1.0mm x 19cm 1.0mm x 22cm 1.0mm x 28cm 1.0mm x 40cm 3.0mm x 14.5cm 3.0mm x 16cm 3.0mm x 17cm 3.0mm x 19cm 3.0mm x 22cm 3.0mm x 28cm 3.
SK16.5-0230 SK16.5-0242* SK16.5-0258* SK16.5-0401 SK16.5-0420 SK16.5-0426* SK16.5-0430 SK16.5-0442* SK16.5-0458* SK16.5-0501 SK16.5-0520 SK16.5-0526* SK16.5-0530 SK16.5-0542* SK16.5-0558* 0.2mm x 30 well 0.2mm x 42 well 0.2mm x 58 well 0.4mm x 1 well 0.4mm x 20 well 0.4mm x 26 well 0.4mm x 30 well 0.4mm x 42 well 0.4mm x 58 well 0.5mm x 1 well 0.5mm x 20 well 0.5mm x 26 well 0.5mm x 30 well 0.5mm x 42 well 0.
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