User Manual

C.B.S.  Scientific 5 Dedicated Height Sequencer
General Information
1.1 Introduction
The C.B.S. Scientific Dedicated Height Nucleic Acid Sequencers feature safety covers with
attached power leads, removable upper and lower reservoirs, epoxy-coated anodized aluminum
heat dispersion plates, buffer drainage ports and a clear acrylic bar clamp. The Dual Dedicated
Height Nucleic Acid Sequencers include the same features, with the convenience of divided upper
reservoirs, if independent runs are required. The Sequencers are available in either a single or
dual format and in 20cm or 33cm widths. Applications include Mutation Detection, Genotyping,
Differential Display, DNA Footprinting, SDS Page, Microsatelites, Rnase Protection and
Sequencing.
1.2 Specifications
Constructions:
Upper buffer chamber Acrylic
Lower buffer chamber/base Acrylic
Safety covers Acrylic
Electrodes Platinum wire .012" diameter
Upright/base Acrylic
Rotating base Acrylic
DC Power leads FR Urethane rated 7500VDC@
200mA, 65ºC
Bar Clamp Delrin and acrylic
Heat dispersion plate Epoxy coated anodized aluminum
Glass plates Soda-lime float glass or
Low fluorescence glass
Combs Polycarbonate, Mylar or White
Spacers Polycarbonate, Mylar or White
White spring clamps Polypropylene
Overall size
Nominal Unit Width 20cm unit 33cm unit
Single unit footprint:
Overall width 30.5cm 41.0cm
Overall height
32cm gel size 41.0cm 43.0cm
42cm gel size 51.0cm 53.0cm
52cm gel size 61.0cm 63.0cm
62cm gel size 71.0cm 73.0cm
Dual unit footprint:
Overall width 30.5cm 41.0cm
Overall height
32cm gel size 41.0cm 43.0cm
42cm gel size 51.0cm 53.0cm
52cm gel size 61.0cm 63.0cm
62cm gel size 71.0cm 73.0cm
Recommended nominal buffer volumes
Single units
upper reservoir 250ml 1500ml
lower reservoir
(per side) 300-500ml 400-600ml
Dual units with Divided Upper Reservoirs
upper reservoir
(total volume/per side) 750/375ml 1500/750ml
lower reservoir
(per side) 300-500ml 400-600ml