User Manual

C.B.S. Scientific 2 Dedicated Height Sequencer
TABLE OF CONTENTS
Page
Important User Information 3-4
Section 1 General Information
1.1 Introduction 5
1.2 Specifications 5
1.3 Safety 6
Section 2 Description of parts
2.1 Unpacking-Dedicated Height Sequencer 7
2.2 Unpacking-Dual Dedicated Height Sequencer 8
Section 3 Assembly
3.1 Unit assembly for Single Dedicated Sequencer 9-10
3.2 Unit assembly for Dual Dedicated Sequencer 10
3.3 Glass Plate Preparation 11
3.4 Methods for Sealing Glass Plate Sandwich 12-13
3.5 Pouring the Gel 14-15
Section 4 Electrophoresis
4.1 Pre-Electrophoresis 16-17
4.2 Loading Samples 17
4.3 Electrophoresis 18
4.4 Post Electrophoresis-Gel Processing 19-20
4.5 Recommended Buffers and Reagents 21
4.6 References and Suggested Reference Literature 22
Section 5 Tables: Power Settings, Troubleshooting, Comb Volumes
5.1 Recommended Power Settings 23
5.2 Troubleshooting Guide 24
5.3 Comb/Well Volumes 25-27
Section 6 Maintenance of Equipment
6.1 Care and Handling 28
6.2 Maintenance 28
Section 7 Ordering Information 29-37