Manual

5
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SECTION 1
General Information
1.1 Introduction
Denaturing Gradient Gel Electrophoresis (DGGE) is a powerful genetic analysis technique
that can be used for detecting single base changes and polymorphisms in genomic (1, 2),
cloned, and PCR amplied DNA (3,4). Two of the most valuable uses for DGGE in human
genetics are in directly detecting single base changes that cause disease and in detecting
polymorphisms with DNA probes for genetic-linkage analysis. Also, DNA fragment melting
points can be determined using Perpendicular DGGE (1).
C.B.S. Scientic has designed four different Cipher DGGEK Systems, which are reliable and
easy-to-use. The DGGEK-1001 is a 2 gel system and features one dual gel cassette. The
DGGEK-2001 features two single gel cassettes. The DGGE-2401 has the same buffer tank
as the DGGE-1001 and DGGE-2001 but features two dual gel cassettes, for a capacity of 4
gels per run. Finally there is the DGGE-4801, which features the larger buffer tank and four
dual cassettes enabling the researcher to run up to 8 gels simultaneously. Improvements to
the technique include: a simplied method for casting perpendicular and vertical gels using
Gel Wrap™. Single or dual gel cassettes have been redesigned eliminating the use of agarose
plugs, the need for an external peristaltic pump for buffer cycling has been eliminated, bulky
screw clamps have been replaced with polypropylene spring clamps, buffer tank dimensions
have been changed to use bench space more efciently, and a safety cover with an electrical
interlock has been added for protection to help maintain temperature and reduce evaporation.
1.2 Specications
Constructions
DGGE Tank Acrylic
DGGE Lid Sodalime glass, acrylic
Electrodes Platinum wire .010” diameter
Power cords FR Silicone rated 7500V, 200mA, 65° C
DGGE Cassette Acrylic
Heater/Stirrer Buffer Cycler Stainless steel
Combs Teon
Spacers UHMW-PE
Glass Plates Sodalime or Borosilicate
Gradient Maker Acrylic, teon, stainless steel
ADDITIONAL SPECIFICATIONS
Model Cat. #
Cipher DGGEK-1001 Cipher DGGEK-2001 Cipher DGGEK-2401
# of cassettes
1 dual cassette 2 single cassettes 2 dual cassettes
Max. # of gels 2 gels 2 gels 4 gels
gel dimensions 15cm(w) x 20cm(l) 15cm(w) x 20cm(l) 15cm(w) x 20cm(l)
Optimal Buffer
Volume
21 Liters -1 dual cassette 21.5 Liters - 1 single cassette
21 Liters - 2 single cassettes
21 Liters - 1 dual cassette
20 Liters - 2 dual cassettes
Minimum
Buffer Volume
Required
18 Liters 18 Liters 18 Liters
footprint (cm) 25.4(w) x 54.6(l) x 34.3(h) 25.4(w) x 54.6(l) x 34.3(h) 25.4(w) x 54.6(l) x 34.3(h)