Manual

26
Cipher DGGE Instructions 2/28/14
4.2 Buffer Cycling Connections-continued.
Buffer Cycling Connections – Dual Cassettes
SECTION 5
Running Conditions
5.1 Running the Gels
1. Load samples at 1:1 with neutral dye. Load 5-10 ug Genomic DNA/well or 1-2 ug
cloned (B-globin)/well. Determine concentration by O.D. 260.
2. Attach black power leads to cassettes as shown in Fig. 5-1.
3. Close lid to engage safety interlock. Turn on power supply to 150V (40mA)
constant Volts for 5-7 hours.
Fig. 5-1
Fig. 4-2e