Owner manual
separation sheet
notched glass plate
spacer
unnotched glass plate
shim sheet
front panel with
captive thumbscrews
casting chamber
lid (used to tilt
assembly)
separation sheet
gure1.
GEL CASTING INSTRUCTIONS
1.Placelidonbenchandputthetopendofthecastingchamber(locatinggrooveprovided)onthelidsothatitrestsat
ananglewiththegasketfacingup.
2.Startbystackingoneseparationsheet,followedbyunnotchedglassplate,spacerset,thennotchedglassplate.
Repeatthissequenceuntilall4sets(or10sets)areneatlystackedintothecastingchamber.Placeoneadditional
separationsheetonthelastglassplate.Seegure1.
3.Placefrontpanelonchamberandtightenthe5captivethumbscrews.With4(or10)setsofglassplateswith0.75mm
spacers,thefrontpanelsealsagainstthegasketandcompressestheglasssandwiches.Usingfewerthan4(or10)
glassplatesetsat0.75mmthickwillrequiretheuseoftheenclosedshimsheetstoachieveatightsealandlight
compressionoftheglassplatesets.Useofotherthan0.75mmthicknessspacersmayrequiretheuseofadditional
shimsheets,whichmustbeorderedseparately.
4.Bringassembledcastertotheverticalposition.Testthetightnessoftheassemblybypullingoneofthepolycarbonate
shimsheets.Ifitcomesouteasily,useextrashimstoincreasetightnessagainstthefrontpanel.
5. Singlepercentagegelscanbecastbyaddingliquidmonomerfromthroughtheluervalveusingapumporlarge
volumesyringe.Forgradientgelsconnectagradientformertoasmallperistalticpump(MPP-100),andthepump
outlettotheluervalvemountedonthefrontpanel.Fillcastertowithin0.5cmoftheglassplatenotches,turnpumpoff
thencloseluervalve.
6.Carefullyinsertcombsintoglassplatesandwiches.Placelidontopofchamberuntilgelspolymerize.
DISASSEMbLy
1.Removelidandcarefullyremoveonecombtoensurepolymerizationiscomplete.Returncastertohorizontalangle
positionasshowningure1.Disassembletheunitbyunscrewingthumbscrews.Releaseeachgelsandwichby
pullingthepolycarbonateseparationsheet.Ifyouareplanningtostoresomeofthegelsforlateruse,leavethe
combinplace,rinseexcessacrylamidefromglassplates,andwrapinSaranwraporstoreinZip-Lockbagsinthe
refrigerator.
2.WashallMulti-CastercomponentsaftereachusewithmilddetergentandrinsewithDIwater.