Owner manual

separation sheet
notched glass plate
spacer
unnotched glass plate
shim sheet
front panel with
captive thumbscrews
casting chamber
lid (used to tilt
assembly)
separation sheet
gure1.
GEL CASTING INSTRUCTIONS
1.Placelidonbenchandputthetopendofthecastingchamber(locatinggrooveprovided)onthelidsothatitrestsat
ananglewiththegasketfacingup.
2.Startbystackingoneseparationsheet,followedbyunnotchedglassplate,spacerset,thennotchedglassplate.
Repeatthissequenceuntilall4sets(or10sets)areneatlystackedintothecastingchamber.Placeoneadditional
separationsheetonthelastglassplate.Seegure1.
3.Placefrontpanelonchamberandtightenthe5captivethumbscrews.With4(or10)setsofglassplateswith0.75mm
spacers,thefrontpanelsealsagainstthegasketandcompressestheglasssandwiches.Usingfewerthan4(or10)
glassplatesetsat0.75mmthickwillrequiretheuseoftheenclosedshimsheetstoachieveatightsealandlight
compressionoftheglassplatesets.Useofotherthan0.75mmthicknessspacersmayrequiretheuseofadditional
shimsheets,whichmustbeorderedseparately.
4.Bringassembledcastertotheverticalposition.Testthetightnessoftheassemblybypullingoneofthepolycarbonate
shimsheets.Ifitcomesouteasily,useextrashimstoincreasetightnessagainstthefrontpanel.
5. Singlepercentagegelscanbecastbyaddingliquidmonomerfromthroughtheluervalveusingapumporlarge
volumesyringe.Forgradientgelsconnectagradientformertoasmallperistalticpump(MPP-100),andthepump
outlettotheluervalvemountedonthefrontpanel.Fillcastertowithin0.5cmoftheglassplatenotches,turnpumpoff
thencloseluervalve.
6.Carefullyinsertcombsintoglassplatesandwiches.Placelidontopofchamberuntilgelspolymerize.
DISASSEMbLy
1.Removelidandcarefullyremoveonecombtoensurepolymerizationiscomplete.Returncastertohorizontalangle
positionasshowningure1.Disassembletheunitbyunscrewingthumbscrews.Releaseeachgelsandwichby
pullingthepolycarbonateseparationsheet.Ifyouareplanningtostoresomeofthegelsforlateruse,leavethe
combinplace,rinseexcessacrylamidefromglassplates,andwrapinSaranwraporstoreinZip-Lockbagsinthe
refrigerator.
2.WashallMulti-CastercomponentsaftereachusewithmilddetergentandrinsewithDIwater.