User Manual

2
Wide Dual Mini-Vertical Instructions 9-30-13
TABLE OF CONTENTS
Important User Information . . . . . . . . . . . . . . . . . . . . . . . .3-4
Section 1: General Information
1.1 Introduction and Features . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Specications and Constructions
. . . . . . . . . . . . . . . . . . . . 5
1.3 Comb Specications for Hand Cast Gels . . . . . . . . . . . . . . . . 6
1.4 Safety
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Section 2: Description of Parts
2.1 Unpacking and Components . . . . . . . . . . . . . . . . . . . . . 7
Section 3: Instructions for Slab Gel Electrophoresis Using Pre-Cast Gels
3.1 Preparing Electrophoresis Unit Using Pre-Cast Gels . . . . . . . . . . . 8-9
3.2 Running the Gel . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.3 Removing the Gel . . . . . . . . . . . . . . . . . . . . . . . . . 11
Section 4: Alternate Protocol for Slab Gel Electrophoresis Using Hand-Cast Gels
4.1 Gel Wrap™ Gasket Casting Method
. . . . . . . . . . . . . . . 12-13
4.2 Preparing Electrophoresis Unit Using Hand-Cast Gels
. . . . . . . . . . . 14
Section 5: Instructions for Western Blotting
5.1 Preparing Unit for Electro-Blotting . . . . . . . . . . . . . . . . . . 15-16
5.2 Electro-Blotting Procedure . . . . . . . . . . . . . . . . . . . . . . 16
5.2 Removing the Blot . . . . . . . . . . . . . . . . . . . . . . . . . 16
Section 6: Running Conditions
6.1 Recommended Power for Slab Gels
. . . . . . . . . . . . . . . . . 17
6.2 Recommended Power for Electro-Blotting . . . . . . . . . . . . . 18
6.3 References . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
6.4 Recommended ClearPAGE™ Buffer Formulation . . . . . . . . . . . . 19-20
Section 7: Maintenance of Equipment
7.1 Care and Handling . . . . . . . . . . . . . . . . . . . . . . . . 21
7.2 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Section 8: Ordering Information
8.1 Ordering Information for Wide Dual Mini-Vertical Electrophoresis Systems . . . 22
8.2 Ordering Information for Related Products . . . . . . . . . . . . . . . 23
Section 9: Contact Information . . . . . . . . . . . . . . . . . . . . . 24