User guide

5
www.cbsscientic.com
SECTION 1
General Information
1.1 Introduction and Features
C.B.S. Scientic offers the Dual Cool Electrophoresis System for performing SDS-Page,
acrylamide-nucleic acid separations and electro-blotting. The dual unit provides the
capability of running or blotting two gels simultaneously under identical temperature
controlled buffer conditions. Units include 2 freezer blocks, 2 blotting cassettes, 2 foam
pads, and a white plastic reservoir conversion plate to allow for single runs.
This system is designed primarily to be used with a variety of precast gels. (See Table
1). Hand-cast gels can also be run in this unit with the use of accessory combs, plates
and spacers.
Table 1: Features of Vertical Mini-Gel Systems
1.2 Specications
Constructions:
Buffer chamber, safety cover, core Polycarbonate/Polysulphone
Electrodes Pure Platinum wire .010” diameter
Power cords Silicone rated 7500VDC,
200mA, 65°C
Combs Teon
Glass plates Soda-lime oat glass & Borosilicate
Spacers PVC
Clamps Polypropylene, stainless steel
Safety Certication EN61010-1-1993 (IEC1010-1)
Table 2: Specications
Model # DCX-700
Shipping Weight 6 lbs
Overall Size W x D x H (cm) 16.5 x 15.2 x 21.6
Recommended buffer volumes:
Cathode reservoir
Anode reservoir
Total buffer volume for 2 blots
200mls
400mls (minimum) / 800mls (maximum)
940mls
Slab gel run time
30-90 minutes
Blot run time
75 minutes
Handcast glass plate size
10cm x 10cm
Voltage limit
300 V
Model # Single or
Dual
Plate Dimensions
(w x h)
Compatible
Precast Gels
Cooling
DCX-700 Dual 10cm x 10cm
ClearPAGE™
Expedeon™
Invitrogen™
1 or 2 freezer blocks
with stirring
10cm x 8cm ClearPAGE™
Expedeon™
10cm x 9cm (requires
adapter #DCX-AP-109)
Lonza™