User Manual

9 Mega-Gel Instructions 12/11
7. Applygelsolutiontogelplatesandwichusinga
syringeorpipette.Ifusingastackinggel,pour
desiredheightofrunninggel,thenoverlayasmall
amountofdH
2
Oor0.1%SDSsolutiontotopof
gel.Afterpolymerization,rinsewithbuffer,add
stackinggelsolutionandinsertcomb.Forregular,
singlepercentagegels,addpolyacrylamidesolution
tocorrectheight,andinsertcomb.Allowgelto
polymerize,usually20-30minutes.Extragel
solutioninpipetteorsyringecanbemonitoredto
testpolymerizationofgelmix.
Leave the comb in the plate sandwich until just
before sample loading
8. Disassembly.Holdtheclampedplateassemblywith
onehand.Removethegasketbystartingatoneofthe
topendsandpulling“upandout”onthegasketuntil
itreleasesfromthe plate, upto thebottom ofeach
ofthe whiteclamps. When eachclamp isreached
DO NOT remove it, instead feed the gasket down
throughtheclampbodyandrepeatpullingupandout.
Continue feeding until the gasket is fully detached.
9. Bottomspacermustberemovedbeforeelectrophoresis.Usinga
spatula,needleorextraspacergentlypushdownonangledpartofbottom
spacerandremove.Ifgelisnottobeusedimmediately,wrapentireplate
sandwichwithplasticwraptightlytosealandstoreat4°Cforuptoaweek.