User Manual

5 Mega-Gel Instructions 12/11
SECTION 1
General Information
1.1 Introduction
This Dual Adjustable Mega-Gel is designed for high-throughput microsatellite mapping (1) with a capacity of up to 200 samples
per run. The glass plate sets include a low uorescence back plate, making it ideal for uorescence-based assays. This feature
allows the bands to be visualized without the time-consuming process of removing the gel from the glass plate sandwich
and staining. Alternatively, ethidium bromide is added to the lower reservoir prior to electrophoresis and the low uorescence
glass allows direct UV visualization and photography of the results without gel removal. To maximize throughput, multiple
amplication products can be loaded simultaneously or successively in the same well (see ref. 1, Wang Paper, pg. 1830).
To further enhance screening productivity, the system includes two 100 well combs that feature microtitre spacing for multi-
channel pipettor loading. This adjustable System offers glass plate sizes of 22cm and 32cm(h).
1.2 Specications
Constructions:
Upper buffer chamber Acrylic
Lower buffer chamber/base  Acrylic
Safety covers    Acrylic
Electrodes Pureplatinumwire.010”diameter
DC Power leads   Silicone7500VDC,200mA,65ºC200mA,65ºC
Glass plates    Soda-limeoatglassorLowuorescentborosilicateglass
Combs    PolycarbonateorMylar
Spacers    Polycarbonate,MylarorPVC
White spring clamps   Polypropylene,stainlesssteel
Table 1: Features of Dual Mega-Gel System C-DASG-400-50
Model# SingleorDual PlateDimensions
C-DASG-400-50 Dual 50cm(w)x22cm(l)
50cm(w)x32cm(l)
Table 2: Specications
Model# C-DASG-400-50
ShippingWeight 2boxes:38lbsand15lbs
OverallSize 34cm(d)x60cm(w)x44cm(h)
Recommendedbuffervolume
Cathodereservoir
Anodereservoir
2literstotal
500mlseachside
500mlseachside
Distancebetweenelectrodes
36cm
RecommendedRunningConditions
350VDC/105mA/gel/1.5mmthickness
1.3 Safety
PowertotheMega-GelunitistobesuppliedbyanexternalDCvoltagepowersupplythatmustbegroundisolated
sothattheDCvoltageoutputoatswithrespecttoground.
Currenttotheunit,providedfromtheexternalpowersupply,mustentertheunitthroughthelidassembly,providing
asafetyinterlocktotheuser.Currenttotheunitisbrokenwhenthelidisremoved. Do not attempt to use the unit
without the safety lid, and always turn the power supply off before removing the lid, or when working with
the unit in any way. Follow safety precautions specied by the power supply manufacturer.