User Manual
12Mega-Gel Instructions 12/11
4.5 References and Suggested Reference Literature
1.Wang,Shi,Carlson,Cregan,Ward,Diers. A Low-Cost, High-Throughput Polyacrylamide Gel
Electrophoresis System for Genotyping with Microsatellite DNA Markers. Crop Science (43:1828-1832)
2. MOLECULAR CLONINGALaboratoryManual,2ndedition,ed.byJ.Sambrook,E.F.FritschandT.Maniatis,
ColdSpringHarborLaboratoryPress,1989.
3. CURRENT PROTOCOLS IN MOLECULAR BIOLOGYed.byF.M.Ausubel,R.Brent,R.E.Kingston,D.D.Moore,
J.G.Seidman,J.A.SmithandK.Struhl,GreenePublishingAssociatesandWiley-Interscience,NewYork,1989.
Section Section 5 Maintenance of Equipment
5.1 Care and Handling
ThecomponentsoftheMega-Gelarefabricatedfromacrylic,Delrin®,Polycarbonate,glassandanodizedaluminum.
Aswithanylaboratoryinstrument,adequatecareensuresconsistentandreliableperformance.
Aftereachuse,rinsebufferreservoirs,combs,spacers,clampsandglassplateswithdeionizedwater.Wipedry
withasoftclothorpapertowel,orallowtoairdry.Whenevernecessary,allcomponentsmaybewashedgentlywith
waterandanonabrasivedetergent,andrinsedanddriedasabove.Neveruseabrasivecleaners,windowsprays
orscouringpadstocleanthecomponents,asthesecausedamagetotheplasticsurfaces.
•Donotautoclaveordry-heatsterilizetheapparatusorcomponents.
•Donotexposetheapparatusorcomponentstophenol,acetone,benzene,halogenatedhydrocarbon
solventsoralcohols.
•AvoidprolongedexposureoftheapparatusorcomponentstoUVlight.
5.2 Maintenance
The following simple, routine inspection and maintenance procedures will help ensure both the safety and the
performanceoftheMega-Gel.Fororderinginformationofreplacementpartscall1-858-755-4959.
•Inspectelectricalconnectionsandpowercordsregularly.Ifthebananaplugconnectorstoeithertheunitorthe
powersupplydonotexhibitreasonablefrictionorcanberockedeasily,replacetheplugsorpowercords.
•Ifpowercordsshowanysignsofwearordamage(e.g.cracks,nicks,abrasions,ormeltedinsulation),replace
themimmediately.
•Examinethebananajackstoensurethattheyaretightandfreeofcorrosion.Ifbananajacksarecorroded,
theyshouldbereplaced.