User Manual

11 Mega-Gel Instructions 12/11
9. ConnecttheDCpowerleadstothepowersupplywiththeproperpolarity.Makesuretheblackleadsareconnected
totheblackcathode(-)andtheredleadsareconnectedtotheredanode(+).
10.Rinsesamplewellswithupperreservoirbuffer.Beforeloadingsamples,pre-electrophoresethegelfor20minutes.
WARNING: Excessivepowerwillcausethegeltooverheatandcracktheglassplates.
4.2 Loading Samples
1. Attheendofthepre-electrophoresisperiod.TURN OFF THE POWER SUPPLY AND DISCONNECT BOTH
DC POWER CORDS FROM THE POWER SUPPLY.Openupperreservoirsafetycover.
2. Immediately prior to loading the samples, rinse the wells of the gel thoroughly with electrophoresis buffer.
Use a  loading tip or a syringe with a bent needle to wash away urea that has diffused into the wells.
4.3 Comb Volume/Maximum well volume
Note:Tocalculatesamplewellvolumeexpressedinmillimeters(mm)dividemaximumvolumebytoothdepth.
-Maximum Volumepertooth(microliters-ml)
#ofwells Tooth
width
0.75mm
thickness
1.0mm
thickness
1.5mm
thickness
100 3mm 22.5ml 30ml 45ml
Overalllengthofcomb:44.8cm
Toothdepth:10mm
Spacingbetweenteeth:1.5mm
4.4 Electrophoresis
1. Afterloadingthesamples,attachthereservoirsafetycover.ConnecttheDCpowerleadstotheunitthentothe
powersupplywiththeproperpolarity.Makesuretheblackleadsareconnectedtotheblackcathodes(-)and
theredleadsareconnectedtotheredanode(+).
2. Turnonthepowersupplyandsetthevoltageorwattagetothepropersettingforthegel. 350V at 105mA/ gel
at 0.5X TBE buffer for 1.5mm thick gel.
3. Monitortheprogressofelectrophoresisbyfollowingmigrationofamarkerdyefrontorbyanyotherpreferred
method.
4. Whenelectrophoresisiscomplete,TURN POWER SUPPLY OFF.Disconnectall DCpowercords,rstfrom
thepowersupply.