User Manual

10Mega-Gel Instructions 12/11
Section 4 Electrophoresis
4.1 Pre-electrophoresis
1. Filllowerbufferreservoirwithbuffer.
2. After removing the Gel Wrap Gasket from the gel sandwich, but with the white clamps still in place,
rinse the sandwich in D.I. water and dry.  Remove comb, then remove the white plastic clamps and
place sandwich (notched plate inward) into the “buffer- lled “ lower resevoir at a slight angle to prevent
capturing bubbles. Reclamp sandwich to unit using bar clamp as quickly as possible as described below.
3. Pre-loosenblackwingnutsonbarclampsoclampassemblyslidesoverglassplatesandupperreservoir.
Tightenwingnutsonlyenoughtocompressgasket.DoNOTovertighten.Seeguresbelow.
4. Liftoutbasesecuringpinandrotatebase180ºforeasyaccesstooppositeside.Repeatsteps1-3withsecond
setofglassplates.
5. Filleachupperreservoirwithapproximately500mlofrunningbuffer.Makesurenobufferisleakingfromtheupper
bufferreservoir.Filleachlowerreservoirwithapproximately500mlofelectrophoresisbuffer.Besurenobubbles
obstructbuffercontactwiththeloweredgeofthegel.Note:DoNOToverllbufferreservoirsorallowbufferto
makecontactwiththebananajacks.
WARNING: No buffer should leak through or around the silicone gasket or down the side of
the gel assembly. Leakage may allow the upper reservoir to drain dry or cause
arcing and damage to the apparatus.
6. Attachthereservoirsafetycoverstotheupperandlowerreservoirs.Connectthefemaleendoftheblackpower
cordintothesafetycoveroftheupperreservoir.Connectthefemaleendoftheredpowercordintothesafety
coverofthelowerreservoir.
7. Connectanexternalchillertothetubingadaptersonthesideoftheaparatusmakingsuretousethebottomtubing
adpaterastheinletandthetopastheoutlet.
8. BesuretoattachTemperatureStrip(Cat.#EGT-100)toupperormiddleofglassplate,tomonitorthetemperature
ofthegel.