Operation Manual Pump Modules C�601 & C�605 Pump Controller C�610 / Pump Manager C�615 96962 en
Table of contents Table of contents 1 2 3 4 5 About this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.1 Reference documents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.2 Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.3 Abbreviations . . . . . . . . . . . . . . .
Table of contents 6 7 8 9 10 11 4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 6.1 Pump system 1: Pump Controller C-610 and 1 Pump Module C-601 . . . . . . . . . 26 6.1.1 Controls of the Pump Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 6.1.2 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1 1 About this manual About this manual This manual describes the Pump Modules C-601 and C-605, the Pump Controller C-610 and the Pump Manager C-615 and provides all information required for its safe operation and to maintain it in good working order. It is addressed in particular to laboratory personnel and operators. NOTE The symbols pertaining to safety (WARNINGS and ATTENTIONS) are explained in chapter 2. 1.
2 2 Safety Safety This chapter highlights the safety concept of the Pump Systems and contains general rules of behavior and warnings from hazards concerning the use of the product. The safety of users and personnel can only be ensured if these safety instructions and the safetyrelated warnings in the individual chapters are strictly observed and followed, therefore, the manual must always be available to all persons performing the tasks described herein. 2.
2 2.4 Safety Warning notices used in this manual WARNING Generally, the triangular warning symbol indicates the possibility of personal injury or even loss of life if the instructions are not followed. WARNING Hot surface. WARNING Electrical hazard. WARNING Biohazard. ATTENTION With the “Read this” symbol, ATTENTION indicates the possibility of equipment damage, malfunctions or incorrect process results if the instructions are not followed. NOTE Useful tips for the optimum operation of the instrument.
2 2.5.2 Safety Other hazards WARNING Certain solvents within or in the vicinity of the Pump Systems can form peroxides and/or are highly inflammable. • A lways be aware of the explosion risk when working with hazardous substances or with substances of unknown composition. • Always use the instrument in an adequately ventilated work area. 2.5.3 Safety measures Always wear personal protective equipment such as protective goggles, protective clothing and gloves when working with the instrument. 2.5.
3 3 Technical data Technical data This chapter introduces the reader to the Pump Systems and its main components. It contains technical data, requirements and performance data. 3.1 Scope of delivery Check the scope of delivery according to the order number. NOTE For detailed information on the listed products, see www.buchi.com or contact your local dealer. 3.1.
3 3.1.2 Technical data Standard accessories Table 3-2: Standard accessories for Pump Module C-601 and C-605 1 Product Order 2 number a Connection cable RJ45, 0.3 m 44288 b Solvent filter 44340 Screwdriver Turix wrench (fits fitting and pipe fitting) - 44304 Green fittings and ferrules, 1/8’’ - White fittings and ferrules, Ø 4.0 mm - FEP pressure tubing 1/8’’ x 1/16’’, 1.5 m - FEP suction tubing 4mm x 2.5mm, length 1.
3 Technical data Table 3-4: Standard accessories for Pump Manager C-615 3.1.
3 Technical data Table 3-6: Optional accessories 8 9 Product Order number h Solvent valve set 44854 i System stand for pump module 44855 j Tubing fitting set 44021 k External pressure sensor without mixing chamber. Needed only for very pressure sensitive columns, enables to measure direct at the column inlet. 54040 Glass column, 15/100 / Prep Elut 44030 Glass column, 26/100 / Prep Elut 44035 10 11 3.
3 3.3 Technical data Technical data overview Table 3-8: Technical data of the Pump Controller C-610 and Pump Manager C-615 Pump Controller C-610 Pump Manager C-615 Dimensions ( W x H x D) 150 x 80 x 120 mm 150 x 80 x 120 mm Weight < 1 kg < 1 kg Connection voltage 24 V DC 24 V DC Function Control unit for isocratic separation with one Pump Module C-601. Simple entry via selector switch and display of flow rate on backlit display.
3 Technical data Table 3-9: Technical data of the Pump Module C-601 and C-605 Dimensions ( W x H x D) 160 x 153 x 305 mm Weight 4 kg Connection voltage 100 – 230 VAC ± 15% Power consumption max.
4 4 Description of function Description of function This chapter explains the basic principle of the Pump Systems and provides a functional description of the assemblies. The design of the pump systems enables the delivery of organic as well as aqueous media without metal contact through a pulsation-poor, 3-piston pump.
4 Description of function The use of two pump modules A and B enables the formation of a binary gradient. The solvents enter the pump heads via the suction tubing (input). The FEP tubing guide the solvents to the mixing chamber with integrated pressure sensor and from there via the injection system on to the column via the pressure tubing (output). The Pump Manager C-615 regulates the flow rate of the pump modules A and B and monitors the current pressure.
5 5 Putting into operation Putting into operation This chapter describes the installation of the Pump Combination and gives instructions on initial startup. NOTE Inspect the instrument for damages during unpacking. If necessary, prepare a status report immediately to inform the postal company, railway company or transport company. Keep the original packaging for future transport. 5.1 Installation site Place the instrument on a stable, horizontal surface and consider the maximum product dimensions.
5 5.3 Putting into operation Installation In order to make things clearer, the following descriptions do not contain the complete names of the modular pump systems, but rather the general name. Pump Controller C-610: Pump controller Pump Manager C-615: Pump manager Pump Module C-601/605: Pump module Mixer (pressure sensor with integrated mixing chamber): Pressure sensor Control Unit C-620: Control unit 5.3.1 Installating the standard accessories Suction tubing 4 mm x 2.5 mm, length 1.
5 Putting into operation Pressure tubing 1/8” x 1/16”: 5 2 1 Cut the pressure tubing to the desired length. Attach the fitting b and the ferrules to one end of the tubing a as described. Screw the fitting b into the upper output e of the separator head. Make sure that all fittings are hand-tight. Fig. 5.3: Pump head with connected pressure tubing 5.3.2 Pump system 1: Pump Controller C-610 and 1 Pump Module C-601 NOTE The Pump Controller C-610 can only be connected to the Pump Module C-601.
5 2 3 4 Putting into operation Connect the pump module a to the pump controller b via data bus RS-485 by means of the cable RJ45 d. c TTL IN/OUT. 1 Fig. 5.6: Rear connections of pump controller and pump module The overpressure sensor switches off the pump if the pressure exceeds 10 bar. This maximum pressure of 10 bar cannot be changed.
5 5.3.3 Putting into operation Pump system 2: Pump Manager C-615 and 1 Pump Module C-601 or C-605 The “or” between 601 and 605 indicates that operation is either possible with Pump Module C-601 up to 10 bar or with Pump Module C-605 up to 50 bar. Place the pump module on a level surface. Connect the power cord. Make sure that the address selector switch is set to A a. If not, take the screwdriver and turn the small white dot to A. Connect the power cord b. 2 1 Fig. 5.
5 Putting into operation Installation of the mixer Click the mixer b to the pump module a. Make sure that all fittings are hand-tight. 3 2 1 Place the mixer cable c on the left side between pump module and pump manager, and attach it to the PRESSURE input of the pump manager (position f in Fig. 5.10). Fig. 5.11: Pump module with attached mixer For information on the installation of the standard accessories (suction tubing), please see chapter 5.3.1.
5 Putting into operation Pressure tubing 1/8” x 1/16”: Cut the pressure tubing to the desired length. Attach the fitting and the ferrules to one end of the tubing as described. 2 Screw the fitting into the upper output of the separator head a. Make sure that all fittings are hand-tight. 1 Inlet is only needed if two pump modules are used. If only one pump module is used it has to be shut with a blind plug. Fig. 5.13: Pump head with connected pressure tubing 5.3.
5 Putting into operation For the installation of the mixer and the suction tubing, see chapter 5.3.3. Connection tubing between the separator heads of the pumps: The fitting and ferrule are attached to both tubing ends. Attach one end of the connection tubing to the input of the lower separator head and the other into the output of the upper separator head . 2 1 2 3 Remove the right rear separator head cover from the lower pump.
5 Putting into operation Pressure tubing 1/8” x 1/16”: Cut the pressure tubing to the desired length. Attach the fitting and the ferrules to one end of the tubing as described. Screw the fitting into the upper output of the separator head. Make sure that all fittings are hand-tight. 3 2 Between the connections a and b runs the connection tubing between the lower head and the mixer. c Outlet mixer mixed solvent 1 Fig. 5.16: Pump head with connected pressure tubing 5.3.
6 6 Operation Operation This chapter explains the operating elements and possible operating modes. It gives instructions on how to operate the Pump Combination properly and safely. ATTENTION Check the glassware for damages prior to each operation and use only glassware in perfect condition. Glassware with cracks, stars or other damages can fail during operation. Make sure that all fittings and tubings are hand-tight. 6.1 Pump system 1: Pump Controller C-610 and 1 Pump Module C-601 6.1.
6 6.2 Operation Pump system 2, 3: Pump Manager C-615 and 1 - 2 Pump Modules C-601/605 Pump Manager C-615 can be operated with one or two Pump Modules C-601or one or two Pump Modules C-605. Since the pump modules only differ with regard to the maximal pressure, they will be described collectively. ATTENTION Note that two Pump Modules of different types must not be mixed. 6.2.
6 1 2 3 4 a Pump status b Current segment 01 c Program 01 5 Operation 6 d Remaining time of current segment e Remaining time of program f Manual mode Fig. 6.3: Overview over the display NOTE When the pump system is pumping, parameters highlighted in a dark color can be changed and selected. Parameters displayed between two black lines cannot be changed anymore (see also picture above). Using the selector switch you can navigate through the menu.
6 6.2.4 Operation Systematics of the Menu Mode Main Menu Flowrate %B * Press. act Press. max. Timer ** Solvent *** Rest-Vol. A1 **** Rest-Vol. A2 **** Rest-Vol. B1 **** Rest-Vol. B2 **** Manual control Show Programs Options Program Menu Back Load Edit View Delete Options Menu Edit Menu Back Language Contrast Pumpe B Valves Pressure Unit Calibration A Calibration B Back Select Pres. max. Flowrate No. of Segments Segment No. Solvent *** %B * Time Save as Fig. 6.
6 6.2.5 Operation Parameters Table 6-1: Functions of the Main Menu Main Menu Description Flow rate Shows currently set total flow rate of eluent (2.5 – 250 ml). %B Shows currently set total flow rate of pump B (0 – 100 %). Press. act. Shows current pressure (0-50 bar) (0 -725 psi). Press. max. Shows selected maximum pressure. Timer 00:00:00 (h:min:sec) If the time limit is zero, the unit is in continuous operation (only in manual operation mode).
6 Operation Table 6-3: Functions of the Edit Menu Edit Menu Description Back Jumps back to Program Menu. Select The programs P01 - P10 or “new” can be selected. Pres. max. Displays the previously set maximum pressure. Flowrate Shows the current eluent flow rate (2.5 – 250 ml) No. of Segments Every program can consist of up to 12 segments. Segment No. Segment numbers can be selected.
6 Operation Konzentration BB(%) (%) Concentration Segment Segment 22 Segment11 Segment Segment Segment 33 End concentration Endkonzentration Start concentration Anfangskonzentration Duration Dauer t (min) TimeZeit t (min) Fig. 6.5: Systematical gradient The program number under which the program is to be saved can be selected at will between P 01 and P 10.
6 Operation Table 6-5: Creating a new program in three steps Step Menu/Confirmation Entry/Confirmation 1 Programs Edit New 2 Select Pres. max. Flow rate No. of Segments Segment No. (automatic) %B Time Segment No. (automatic) %B Time Segment No. (automatic) %B Time --> Save New 50 bar 10 ml 3 1 0-0 00:25:00 2 0 - 35 00:30:00 3 35 - 35 00:20:00 P02 The program is loaded automatically 3 Show Gradient is shown NOTE When your presettings change your programs might make no sense anymore.
6 6.4 Operation Calibration ATTENTION Before carrying out the calibration make sure that the system is free of air. Always calibrate the system under your actual working conditions (flow rate, backpressure, column). After calibration carry out the measurement again to check the current pump flow rate. The pump flow rate can be influenced by the following factors: • • • • • 6.4.1 The length of the suction tubing. The location of the solvent vessel above or below the pump. The viscosity of the solvent.
6 6.4.2 Operation Calibration of the Pump Manager C-615 Requirements: To calibrate the Pump Manager C-615 you need a graduated cylinder and a stopwatch. Set the pump flow rate to 100 ml/min and the time to one minute. Place the pressure tubing into the graduated cylinder and start the pump. If the cylinder contains exactly 100 ml, the pump is calibrated. If too little or too much solvent is in the cylinder, the flow rate is too low or too high. If this is the case, recalibrate the pump.
7 7 Maintenance Maintenance This chapter provides instructions on all required maintenance to keep the instrument in good working condition. WARNING All maintenance and repair work requiring the opening or removal of instrument covers and lids must be carried out by trained personnel and only with the tools provided for this purpose. WARNING Electrical hazard: • P rior to any maintenance work on the instrument switch off the power supply and remove all sources of flammable vapor.
7 7.5.1 Maintenance Cleaning the seals To prolong the lifetime of the seals and in case of unwanted sample contamination (foaming or boiling retardation) rinse the seals with water or ethanol. Dry the cleaned seals with a soft cloth. 7.5.2 Changing the seals Visually examine the seals regularly, if seals become cracked and brittle replace them with new ones. For this purpose, proceed as follows: Remove the pump head cover. Loosen the pump head screws and remove it.
7 7.6 Maintenance Piston backrinsing ATTENTION When working with aqueous saline solutions, residues might collect behind the piston seal. These residues must be removed since they can contribute to a faster wearing of the seals. The system can be rinsed with a spraying device filled with water. We recommend to use a tissue at the outlet of the backrinsing to collect the water drops. Fig. 7.3: Piston backrinsing with spraying device and tissue 7.
7 2 1 Fig. 7.5: Installing the new valves 7.8 Maintenance • Remove the valve screw fittings and tubing. • Remove the valves and insert the new valves. The inlet valve is marked by one ring a at the head end, and is attached with that ring facing the pump head in the direction of the arrow. The outlet valve is marked by two rings b at the head end and is inserted into the pump with the unmarked side first. The valves are positioned loosely in the pump head.
8 8 Troubleshooting Troubleshooting The following chapter describes how to resume operation of the instrument in the event of any minor problem. It will list some possible occurrences, their probable cause and suggests how to remedy the problem. The troubleshooting table below lists possible malfunctions and errors of the instrument and describes operator enabled courses of action to correct some of tubing problems by him or herself.
8 Troubleshooting Table 8-1: Error messages of the Pump Controller C-610 (cont.) Error number Possible cause Remedy 08 Invalid data in pump electronics EEPROM. Contact the BUCHI customer service 09 RS-485 bus voltage too low. Contact the BUCHI customer service 10 Internal auxiliary voltage not within tolerance of pump electronics. Contact the BUCHI customer service 11 Pump electronics fan defective. Contact the BUCHI customer service 23 EEPROM C-605.
8 Troubleshooting Table 8-2: Error messages of the Pump Manager C-615 (cont.) Error number 8.2 Possible cause Remedy 22 Cooling fan in pump B not working. Contact Customer Service. 23, 24 EEPROM C-615. Contact Customer Service. 26 Pressure higher than max. pressure settings. Lower the flowrate, if possible, increase the max. pressure 27 Unexpected restart. Restart. 28 Check solvent level A. Refill solvent bottle A. Check level sensor position. Check level sensor adjustment.
8 Troubleshooting When the measures above are not successful, continue as follows: • Localizing the defective valve: –– Let the pump module run with low flow rate (10 ml/min) and pull the suction tube out of the solvent bottle for a short time (about 1 second). –– Observe, at which of the three suction tubes the air bubble does not disappear after 1 minute. –– Switch off the pump. –– Dismount the inlet valve. Make sure that the thin PTFE sealing does not get lost.
9 9 Shutdown, storage, transport and disposal Shutdown, storage, transport and disposal This chapter instructs on how to shut down the instrument, how to pack it for storage or transport and specifies the storage and shipping conditions. 9.1 Storage and transport WARNING Biohazard: • R emove all dangerous substances from the instrument and clean it thoroughly by flushing it with dry air or nitrogen. Store and transport the instrument in its original packaging.
10 10 Spare parts Spare parts This chapter lists spare parts, accessories, and optional extras, including all of the relevant order information for ordering from BUCHI. Always state the product designation and part number when ordering any spare parts. Use only genuine BUCHI consumables and spare parts for maintenance and repair to ensure optimum system performance and reliability. Prior written permission of the manufacturer should be obtained before any modifications are made to the spare parts used.
10 Spare parts Table 10-1: Spare parts (cont.) 5 46 Product Order number e Mixer (pressure sensor with integrated mixing chamber) 51100 Overpressure sensor 51110 FEP pressure tubing 1/8’’ x 1/16’’, length 5 m 44354 FEP suction tubing 4mm x 2.
11 11 Declarations and requirements 11.1 FCC requirements (for USA and Canada) Declarations and requirements English: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to both Part 15 of the FCC Rules and the radio interference regulations of the Canadian Department of Communications. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
11 11.
BÜCHI Labortechnik AG CH-9230 Flawil 1 / Switzerland T +41 71 394 63 63 F +41 71 394 65 65 www.buchi.
