Operation Manual Hydrolysis Unit B�411/E�416 96685 en
Table of contents Table of contents 1 2 3 4 5 About this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.1 Abbreviations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 2.1 User qualification . . . . . . .
Table of contents 6 7 8 9 10 4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 6.1 Sample weight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 6.2 Preheating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 6.3 Preparing the digestion vessels . . . . . . . . . . .
1 1 About this manual About this manual This manual describes the Hydrolysis Unit B-411/E-416 and provides all information required for its safe operation and to maintain it in good working order. It is addressed to laboratory personnel in particular. NOTE The symbols pertaining to safety (WARNINGS and ATTENTIONS) are explained in chapter 2. 1.
2 2 Safety Safety This chapter points out the safety concept of the instrument and contains general rules of behavior and warnings from hazards concerning the use of the product. The safety of users and personnel can only be ensured if these safety instructions and the safetyrelated warnings in the individual chapters are strictly observed and followed. Therefore, the manual must always be available to all persons performing the tasks described herein. 2.
2 2.4 Safety Warning notices used in this manual WARNING Generally, the triangular warning symbol indicates the possibility of personal injury or even loss of life if the instructions are not followed. WARNING Hot surface. WARNING Electrical hazard. ATTENTION With the general “Read this” symbol, ATTENTIONs indicate the possibility of equipment damage, malfunctions or incorrect process results, if instructions are not followed. NOTE Useful tips for the easy operation of the instrument. 2.
2 2.5.3 Safety Safety elements • T he main switch of the instrument serves as safety switch. In case of overload or short circuit it automatically falls back to the zero position. 2.6 General safety rules Responsibility of the operator The head of laboratory is responsible for training his personnel. The operator shall inform the manufacturer without delay of any safety-related incidents which might occur during operation of the instrument.
3 3 Technical data Technical data This chapter introduces the reader to the instrument specifications. It contains the scope of delivery, technical data, requirements and performance data. 3.1 Scope of delivery Check the scope of delivery according to the order number. NOTE For detailed information on the listed products, see www.buchi.com or contact your local dealer. 3.1.
3 3.1.2 Technical data Standard accessories Table 3-2: Standard accessories 5 3 6 2 4 1 10 B-411 E-416 Product Order number 1 2 a Set of 4 digestion vessels, 300 ml 37377 4 6 b Sample aspiration tubes 37380 1 - c Set of 4 caps for rinsing digestion vessel 37463 - 6 Cap for rinsing digestion vessel 19741 1 2 d Set of 4 stoppers, Ø 45 mm 37725 1 1 e Inbus wrench 3.0 mm 00610 4 6 f Rubber coupling, EPDM 37381 4 - Rubber coupling, Viton 44422 1 1 Bottle of 2.
3 Technical data Table 3-2: Standard accessories (cont.) B-411 E-416 1 1 a 1 3.1.
3 3.2 Technical data Technical data overview Table 3-4: Technical data Hydrolysis Unit B-411/E-416 Dimensions (W x H x D) 275 x 600 x 570 mm Weight B-411: 13.5 kg E-416: 14.5 kg 3.
4 4 Description of function Description of function This chapter explains the basic principle of the instrument, shows how it is structured and gives a functional description of the assemblies. For Weibull-Stoldt fat determination in food and animal feed, the product must be hydrolyzed to make the fat accessible for extraction. Thus a reproducible fat determination is possible.
4 4.2 Description of function Description of the operating elements a Main/safety switch b Energy regulator c Heating indicator lamp 3 1 2 Fig. 4.2: Operation panel B-411 4.3 Principle of hydrolysis The sample is boiled in diluted hydrochloric acid to break down proteins and high-molecular carbohydrates into acid-soluble constituents. Fig. 4.3: Boil position Afterwards the sample is filtered over a prepared glass sample tube and dried.
5 5 Putting into operation Putting into operation This chapter describes how the instrument is installed and gives instructions on initial startup. NOTE Inspect the instrument for damages during unpacking. If necessary, prepare a status report immediately to inform the postal company, railway company or transportation company. Remove the transportation lock at the bottom of the instrument. Keep the original packaging for future transportation. 5.
5 5.4 Putting into operation Installing the rubber couplings The rubber couplings can be carefully installed on the suction tube and the sample aspiration tube after having moistened them with water. During the installation, make sure that the collar of the glass part abuts on the receptacle of the rubber part. The receptacles for the glass sample tubes are provided with an antistick coating, so that they can be easily installed and disassembled.
6 6 Operation Operation This chapter gives examples of typical instrument applications and instructions on how to operate the instrument properly and safely. WARNING Risk of injury. • Never operate the instrument when glassware is damaged. ATTENTION Hot, caustic hydrochloric acid is used for the operation of the instrument. For this reason, personal protective equipment must be worn at all times when using this instrument or working in its immediate proximity. 6.
6 Operation Add 50 ml HCl, 4 mol / l and shake the tube to mix the sample with the celite and the HCl. Fig. 6.3: Adding HCI and mixing Add another 50 ml HCl, 4 mol / l by pouring it along the side walls of the digestion vessel to remove probable residues from the tube wall. In case of liquid samples, the acid concentration should be adjusted to the water content of the sample. Fig. 6.4: Adding HCl to rinse glass wall 6.4 Preparing the glass sample tubes Evenly distribute approx.
6 6.5 Operation Choosing the heat output When the preheating procedure is finished, the hydrolysis can be started. For this purpose we recommend to switch the energy regulator to position 2 for four samples and to position 3 for six samples. Depending on the boiling behavior, less heat can be provided when setting the regulator to position 1 or more heat can be provided by setting it to position 3. 6.
6 6.8 Operation Drying the hydrolyzed samples Before drying the hydrolyzed samples stir the sample layer and the celite layer (without touching the sand layer) with a spatula to loosen the pulp. Afterwards wipe the spatula with fat free cotton wool or a tissue and put the tissue into the sample. Dry the glass sample tubes in a vacuum oven (≤ 4 h at 100°C/200mbar), in an oven (≤ 8 h at 100°C) or in a microwave oven. Using a microwave oven makes the drying much faster.
7 7 Maintenance and repairs Maintenance This chapter gives instructions on all maintenance work to be performed in order to keep the instrument in good working condition. WARNING All maintenance and repair work requiring the opening or removal of instrument covers may only be carried out by trained personnel and with the tools provided for this purpose. WARNING Electrical hazard: • P rior to all maintenance work on the instrument switch off the power supply and remove all sources of flammable vapor.
7 7.3 Maintenance and repairs Glass parts The glass parts can be taken out and cleaned with commercially available cleaning agents or in an ultrasonic bath. After the glass parts have been cleaned and fully dried, check each part visually for cracks, scratches and for any parts or sections that might have splintered off. Take out and replace any damaged glass parts. 7.4 Rubber couplings Rinse the rubber couplings regularly with water. 7.
7 7.7 Maintenance and repairs Customer service Only authorised service personnel is allowed to perform repair work on the instrument. These persons have a comprehensive technical training and knowledge of possible dangers which might arise from the instrument. Addresses of official Buchi customer service offices are given on the Buchi website under: www.buchi.com. If malfunctions occur on your instrument or you have technical questions or application problems, contact one of these offices.
8 8 Shutdown, storage, transport and disposal Shutdown, storage, transport and disposal This chapter instructs how to shut down the instrument, how to pack it for storage or transport, and specifies the storage and shipping conditions. 8.1 Storage and transport WARNING Unplug the mains cable and remove and thoroughly rinse the suction tube. Clean the instrument thoroughly, remove all chemical residues and wash the glass parts. Store and transport the instrument in its original packaging. 8.
9 9 Spare parts Spare parts This chapter lists spare parts, accessories, and options including their ordering information. Order the spare parts from Buchi. Always state the product designation and the part number when ordering spare parts. Use only genuine Buchi consumables and genuine spare parts for maintenance and repair to assure good system performance and reliability. Any modifications to the spare parts used are only allowed with the prior written permission of the manufacturer.
10 10 Declarations and requirements 10.1 FCC requirements (for USA and Canada) Declarations and requirements English: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to both Part 15 of the FCC Rules and the radio interference regulations of the Canadian Department of Communications. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
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BÜCHI Labortechnik AG CH-9230 Flawil 1 / Switzerland T +41 71 394 63 63 F +41 71 394 65 65 www.buchi.
