Owner's manual

6 Operation
41 E-812/816 Soxhlet Operation Manual, Version B
6. Place the beakers on the corresponding positions directly on the heating plate.
7. Lower the rack.
8. Activate the occupied positions.
9. Select a previous program or enter a new one.
10. Open the cooling water flow or switch on the connected chiller.
11. Start the extraction process.
6.4.3 After the extraction process
The end of an extraction process is indicated on the display and audible by a beep sound (if selected
in the instrument settings).
After an extraction process is finished, proceed as follows:
• Confirm the message on the screen by pressing the backward button.
• Empty the solvent tank.
• Move up the rack.
• Carefully remove the glass tubes or thimbles from the beakers by means of the pliers.
• Place the glass sample tubes or thimbles on a tissue and let them dry under the fume hood.
• Dry the beakers in a drying oven at 100 °C or in a vacuum oven at 200 mbar and 70 °C until they
have a constant weight.
• Let the beakers cool down to ambient temperature in a desiccator.
• Weigh the beakers and calculate the resulting fat content.
6.4.4 Emptying the solvent tank
• To empty the solvent tank put the solvent outlet hose into the waste container.
• Press Solv. and confirm the appearing message by pressing Yes.
NOTE
Make sure to empty the solvent tank after each extraction.
6.4.5 Reusing the solvent
• The solvent can be reused directly or after being distilled in a rotary evaporator.
When the instrument was used for the first time:
• Waste the solvent
When a different solvent was used before
• Waste the solvent
After incorrect procedure:
• Clean the glass assembly
• Clean the solvent tank
• Waste the solvent
NOTE
When working with petroleum ether from 40 - 60 °C: Renew the whole solvent after 10 extractions
to achieve a reproducible extraction behaviour. The mixture changes within time because the light
fraction evaporates and the heavy fraction accumulates.