Manual

5 Putting into operation
31 B-395 Pro Operation Manual, Version C
5.5.2 Pressure bottle
The pressure bottle is an autoclavable container used to pump the immobilization mixture by air pres-
sure. Figure 5-17 shows the different parts of the pressure bottle.
1 3 42
67
5
89
Figure 5-17: Pressure bottle with HEPA filter for sterile pumping of the immobilization mixture with air pressure
a Pressure stable flask of 500 mL or 1,000 mL
HEPA air filter
PTFE tube (4×6)
Silicone tube for liquid (4×7)
Silicone tube for air (5×8)
Luer lock male, 4.8 mm ID
Nipple for quick coupling
Two port cap
Cap with PTFE fitting for 6 mm tubes
The air passes through a silicone tube with an inner diameter of 5 mm (5×8 mm). The Hepa-filter
prevents contamination of the sterile immobilization mixture and should be replaced according to the
manufacturer’s instructions or if signs of reduced air passage are noticeable.
The liquid passes from the inside of the bottle through a PTFE tube (3×6 mm) to the silicone tube
outside of the bottle. This silicone tube is attached to the bead producing unit with the luer lock male .