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TECHNICAL NOTE

APPLICATION

KNOWLEDGE

Liquid Handling with Bottle-Top Instruments:

Solutions for special applications

Author: Dr. Patrick Jost

BRAND GMBH + CO KG, Wertheim

Bottle-top dispensers and bottle-

top burettes enable liquid han-

dling directly from bottles, canis-

ters or other containers.

The bottle-top concept saves time

in the laboratory and ensures

greater safety since no intermedi-

ate steps are required. For many

laboratory applications, bottle-top

instruments can be used univer-

sally.

In this technical note, we present

solutions for applications that are

particularly challenging due to the

media or container being used.

The Dispensette®, seripettor®, and Titrette® are attached to a container of

medium via a GL45 internal thread. The connection element on liquid handling

instruments is called a valve block adapter and this is firmly connected to the

instruments. The GL45 thread is a common size for glass or plastic laboratory

bottles from many dierent manufacturers. Laboratory chemical suppliers oen

use bottles with a smaller thread diameter, such as 40 mm. Plastic canisters,

on the other hand, oen have threads with larger diameters, such as 50 mm or

60 mm. In order to attach BRAND bottle-top instruments to a variety of dierent

media containers, bottle adapters that adapt the GL45 thread of the instruments

to the media container thread are required. To determine the thread size of a

bottle and choose the right bottle adapter, the outer diameter of the thread (A)

and the thread pitch (B) are measured.

Another characteristic of a thread is its shape. GL-threads are round, while

S-threads have the shape of a saw tooth. However, due to the tolerances,

S- and GL-threads are usually compatible with one another. Alternatively,

BRAND provides a document on its website with adapter templates.

Introduction Bottle adapters

Determining the thread size of a bottle

Summary of content (6 pages)

PAGE 1
TECHNICAL NOTE APPLICATION KNOWLEDGE Liquid Handling with Bottle-Top Instruments: Solutions for special applications Introduction Bottle adapters Bottle-top dispensers and bottletop burettes enable liquid handling directly from bottles, canisters or other containers. The bottle-top concept saves time in the laboratory and ensures greater safety since no intermediate steps are required. For many laboratory applications, bottle-top instruments can be used universally.
PAGE 2
TECHNICAL NOTE BRAND offers a selection of thread adapters for common thread sizes in the laboratory. For bottle adapters with a bottle thread diameter less than 32 mm, the bottle adapter GL 32 – 33 (Item No. 704396) must be inserted. One possible combination would be Dispensette® S (valve block adapter GL45) on bottle adapter GL 32 – 33 (Item No. 704396) on bottle adapter GL 28/ S 28 (Item No. 704328) on a media bottle with GL 28 thread. You can find our adapter templates at shop.brand.
PAGE 3
TECHNICAL NOTE Drying tube Bottle-top dispensers and bottle-top burettes come with a ventilation channel, which ensures that the pressure inside the media bottle is the same as the ambient pressure. If the inside of the bottle were to be hermetically sealed from the ambient atmosphere, a vacuum would be created in the bottle as removal of the medium increases. Depending on the vapor pressure of the medium, it would begin to evaporate as a result.
PAGE 4
TECHNICAL NOTE Flexible discharge tubing Work efficiently with flexible discharge tubing When dispensing media with bottle-top dispensers, the sample cup is usually moved into position at the tip of the discharge cannula. In the case of small-volume sample containers, such as test tubes or cuvettes, which are placed in stands for better handling, it is advisable to do the opposite. This is where flexible discharge tubing comes in handy, as it can be moved to the sample container.
PAGE 5
TECHNICAL NOTE Dispensette® drum extraction system When you want to dispense media directly from drums or canisters, you are faced with two problems: 1. This uses up a lot of space on the worktable. 2. The Dispensette® is positioned quite high above the work table, which often makes it difficult to work ergonomically. The Dispensette® drum extraction system provides a solution in this situation.
PAGE 6
BRAND GMBH + CO KG P.O. Box 1155 | 97861 Wertheim | Germany T +49 9342 808 0 | F +49 9342 808 98000 | info@brand.de | www.brand.de BRAND®, BRAND. For lab. For life.®, Dispensette®, Titrette®, as well as the BRAND figurative mark are registered trademarks or trademarks of BRAND GMBH + CO KG, Germany. All other trademarks mentioned or depicted here are the property of the respective owners. Our technical literature is intended to inform and advise our customers.