Introduction We would like to express our gratitude for your purchase of our autoclave. This brochure covers the operation method and a simple maintenance method for the Autoclave HV-25/50/85/110 you now own. We hope that owing to your proper handling the autoclave can demonstrate its full performance and that you will make regular use of it for a long time.
Before Using In this manual the following headwords are applied to items to which great attention should be given: WARNING: Precaution indicating an imminent dangerous situation which if not avoided may lead to death or serious injury. a CAUTION: Precaution indicating a dangerous situation which if not avoided may lead to moderate or slight injury. IMPORTANT: Indicates items you are strongly advised to obey. NOTE: Items you should pay attention to during operation.
(5) Harnmable gas (hydrogen, acetylene, ethylene, methane, ethane, propane, butane, and other substances that are gas at a temperature of 15°C at 1 atmospheric pressure.) Before opening the lid, be sure to check that the pressure gauge reads "0 kgf/cm2". I 0 - Never modify the product. A CAUTION Foreign matter (metals, liquid) may enter through the vent hole. Operating the equipment with such foreign matter inside may cause trouble with the equipment, fire and electric shock.
Do not unload the exhaust bottle or drain the working chamber when the chamber is under pressure. Boiling water or steam may gush out, and you may get burned. a Do not unload the exhaust bottle before water in the bottle has been sufficiently cooled. 0 Do not dispose of used batteries in fire; they may explode. a If you encounter any anomaly (e.g. abnormal sound and smell, smoking), immediately shut off power.
How to Read This Manual a This operation manual consists of the following sections covering the information required for proper operation of the autoclave HV-25/50/85/110: Chapter 1. What Is Autoclave HV-25/50185/11O?" This section describes the uses and features of the product and the names and functions of its parts. Chapter 2. Installation This section explains where the equipment should be installed and how to install it. The product incorporates precision parts.
Contents Introduction .................... . ............................................................................................ Table of Contents ......................................................... ............ Before Using ............................................................................................................... How to Read This Manual .................... . . . ................................................................. Contents ..................... . . ........
C h a ~ t e 1. r What Is Autoclave HV-25/50/85111O? 1. Product Uses This product is designed for research use only (Modes 1 - 3). It is not labeled or marketed for use in medical or clinical applications. This product is also used to liquefy media (Mode 4). Mode 4 can also be used to prewarm the chamber for a faster start. 2. Product Features This product is equipped with a cover for the lid that may be heated at hlgh temperature during use.
Display and Operation Switches @ Digital Display (Temperature, Time,of Day, Error) The digital display indicates set temperature, current time and pre-selected time of day when the equipment is in a standby state and it indicates temperature in the working chamber during operation. If any troubIe occurs in the equipment and an error is detected, the display indicates the error.
Chapter 2. Installation - A CAUTION If the equipment is installed in a place which is 800 m or higher than sea level (i.e. under low pressure in mountain areas), its specifications require to be changed. In this case, be sure to contact us. Do not use the equipment before changing. In transporting the equipment, put the lid on and slide the opedclose lever to the end of the close side to prevent the lid from opening.
2, Installation Procedure @ Put the body on the caster stoppers to prevent the body from ac Anchor the body as described in the following. (1) Set the stopper the specified distance from walls. Specified distance L = HV-25 .............. 49 cm or over HV-50 .............. 55 cm or over Push HV-85/110 ....... 67 cm or over (2) Push the body until the front casters roll onto the stopper. @ Connect the power cord to a rated power supply. Reliably ground the earth cable.
@ Pour water into the exhaust bottle. Add water to the exhaust bottle as described below. (1) UnIoad the exhaust bottle from the body. Pull the bottle outwards until the top handle can be grabbed securely. Lift the bottle out of the autoclave using this handle. Drain po Pol (2) Pour water into the bottle through the water filling port. Fill water to the reference line level. Reference line level (3) Check to make sure that the water level is at the LOW level (the lowest water level).
@ Referring to "chapter 3. Operation Method," open the lid and take out the accessories. @ Place the bottom place in the working chamber. @ When the product is operated continuously, steam may be emitted from the exhaust pon located at the rear of the body. In this case connect one end of the attached exhaust hose to the exhaust port and place the other end in a container. Discard water collected in the container to prevent the end of the exhaust hose from becoming immersed in water.
Chapter 3. Operation Method Basic Operation Method 1 Turning "ON' Power Switch See '1. Turning 'ON' POWER Switch" on page 8. v Open the lid. I 1 v See '2. Pouring Water" on page 8. Pour water. v i Load substance. A- 1 1 v 1 Put the lid down. i Check and select mode. I Check and change set values. I I i i See "4. Selecting Mode (Process)" on page 10. 1 --------- See '5. Changing Set Values" on page 12. When starting operation by Turn-on Timer 1 I I Set Tum-on Timer.
1. Turning "ON" POWER Switch O Turn ON the POWER switch at the front of the body. When the openiclose lever is set to "LOCK" (left side), settings light up on the display. The autoclave is ready in this state. When the open/close lever is set to "UNLOCK" (anywhere other is shown on the display. than on the left side), " L Ready state 2. Pouring Water ,. IMPORTANT 0 In operation of UNLOCKLOCK lever, never fail to put POWR switch ON. a Slide the open/close lever to the UNLOCK side (the right end).
3. Loading Substance I Be careful not to pinch hands when closing the lid. Check to see that the area of the lid that is to come in contact with the lid gasket is free from foreign matter before closing the lid. Foreign matter present in the area may cause the steam leakage. a Whenever a bag, such as a waste disposal bag, is used for sterilization, put it in the wire mesh basket and place the basket in the working chamber.
Do not pile specimens on top of one another. When the chamber is overly packed, steam fails to penetrate to all points, resulting in incomplete sterilization. 0 In sterilizing liquids such as chemical and medium, pay attention to the quantity of the liquid in relation to its container. For an Erlenmeyer flask, the amount of chemical should be approx. 314 of the capacity of the container; for a test tube, the appropriate quantity of chemical is approx. half of the capacity of the container.
Initial Set Value Sterilization Sterilization Warming Exhaust % Temperature Time Temperature Step Display WEATG -+ STER. -+ Mode 1 12 1 OC EXHT.(Varied) -+WARM Step Display BEATG -+ STEW. -+ EXHT. (Varied) Mode 20 minutes 50 "C 10% Initial Set Value Sterilization Sterilization Warming Exhaust % Temperature Time Temperatul~ 10% 20 minutes 121 OC / XI-J- IVIUUC Display ILJQ Mode ~ i ~ L ~ Q I 2 Alternate display Step Display HEATG -+ STER. -+ EXHT.
5. Changing Set Values (Registering Values by Customer) To change set values (sterilization temperature, sterilization time, warming temperature, opening of exhaust valve, liquefaction temperature, liquefaction time), follow the steps below: Settings cannot be changed while the chamber is running except if wanting to change the exhaust rate while exhausting the chamber.
Sterilization time: 1 minute increments within a range of 1-250 minute Liquefaction temperature: In increments of 1°C within a range of 60100°C Liquefaction time: 1 minute increments within a range of 1-60 minutes Warming temperature: 1°C increments w i t h a range of 45-60°C EXHT %: 1% increments within a range of 0-100% If you hold the switch down, the displayed value increases or decreases in 10 unit increments.
I I If steam is abruptly exhausted after sterilization of liquid, the liquid may gush out. To prevenl this, set the opening of the exhaust valve (Exhaust %) to a small value to gradually exhaust. Or, set that to 0% (spontaneous cooling). When used with a sterilization can, it takes several hours for the temperature in waste disposal bag to reach the set temperature after the temperature in the working chamber (displayed temperature) reaches the set value (time lag). If there is approx.
6. Checking and Correcting the Clock Time @ With the equipment in a standby state, press the FUNC. switch twice. The CILOCK display lights up, and the current time appears on the digital displays. O Check to see that the displayed time is correct. If the time is correct: Press the MODE switch. (Returns to a standby state) If the time is incorrect: Go to O. O Press the SETENT switch. The MONTH display starts flickering. This indicates that the clock is now ready for correction.
8 Press the SETIENT switch. The corrected current time is stored, and the equipment returns to a standby state. Canceling Correcting Clock If halfway you desire to cancel correction of the clock, press the MODE switch. The changed current time is not stored, and the equipment returns to a standby state. r /\ NOTE The clock continues working by means of a backup battery even after the power switch is turned off, When the backup battery has run down, the QIILOCKdisplay flickers.
7. Checking and Setting of Turn-on Timer a IMPORTANT iI 0 Invalid setting of the turn-on timer and an incorrect clock may lead to equipment malfunctions. Be sure to check the clock and correct it if necessary before setting the turn-on timer With the equipment in a standby state, press the FUNC. switch once. The TIMER display lights up, and the default value of the turn-on timer (the initial set value of operation start time) will appear on the digital display.
Example of Setting: When you desire to start operation at 11:30 p.m. today, your setting should be as follows: Day(s)-later setting = 0, Hour setting = 23, Minute setting = 30 When you desire to start operation at 5:00 a.m. the day after tomorrow, your setting should be as follows: Day(s)-later setting = 2, Hour setting = 5, Minute setting = 0 O Press the SET/ENT switch. The set turn-on time is stored, and the equipment returns to the standby state with the Tl[MIEK display on.
8. Starting Operation O Check again to see that the water level in the exhaust bottle is between the HIGH and LOW levels. If above the HIGH level ... See "2.Draining Working Chamber"on page 27. If below the LOW setting ... See "2. Installation Procedure O" on page 5. O Press the STARTISTOP switch. The open/close lever is locked, and it is become impossible to open the lid. Thereafter, one of the following processes is performed depending on the chosen mode of operation.
- Exhaust % During the Valve Opening Variable Exhaust Cycle O Press the SETIENT switch. The display of opening of the exhaust % display starts flickering, and indicates that it is now ready to be changed. O Change the numeric value using the set value increasddecrease switches (A,.I(.). Each time you press the switches, the displayed value increases or decreases in 1% increments within a range of 1-100%. If you hold down the switches, the displayed value increases or decreases in 10% increments.
Unloading 9. A WARNING Check to see that the gauge for pressure in the working chamber reads 0 kgf/cm2. - A CAUTION D o not put your face or hands close to the working chamber when lifting the lid after operations are complete; steam will gush out of the opening of the chamber. After operation is over, the lid, chamber, packing and panel are hot. To protect yourself from bums, do not touch with bare hands. It takes a lot of time for the liquid to cool.
11. To Interrupt Operation A NOTE If operation is interrupted during the sterilization of liquid, the liquid may overflow into the worlung chamber. I O Press the STARTISTOP switch. The ongoing process will be interrupted, and the equipment returns to the standby state (state before operation). When you take the sterilized substance out of the working chamber, follow the instructions described in "9. Unloading".
Any air remaining in the working chamber makes the temperature distribution in the chamber uneven. This hinders temperature rise (sterilization). To cope with this, a microcomputer-controlled automatic exhaust valve vents virtually 100% of the air. Temperature rises until a set sterilization temperature (pressure) is reached. After the set sterilization temperature is attained, the WIEATG stops flickering and lights up. Operation proceeds to the next step.
When the pressure in the working chamber falls below 0.1 kgf/cm2, the exhaust valve is fully opened. When the temperature in the working chamber (or the temperature resistered by the floating sensor, if used) falls to 97°C (or 7g°C)*, the EWWT. display stops flickering and lights up. The display of opening of the exhaust valve (exhaust %) disappears from the digital display, and operation proceeds to the next cycle. * Specifications for particular area.
Completion Cycle --- Common to all the Modes @When all cycles of each mode are complete, the electronic alarm indicates completion of all cycles by giving a beeping sound three times. Then the COMP.display goes out and starts flickering.
Chapter 4. Maintenance and Service r I Be sure cAuT1oN to start maintenance or service work after the main body has been sufficiently cooled. 1. Draining Exhaust Bottle Water collects in the exhaust bottle as operations are repeated. If the water level is above the HIGH level (allowable highest water level), drain the bottle in accordance with the following procedures. ; A Do not unload the exhaust bottle before water in the bottle has been sufficiently cooled.
-- - - - - - --- 2. Draining Working Chamber When you plan to put the equipment out of service for a long time, be sure to drain the working chamber to prevent the clogging of pipes. After checking that the working chamber has been sufficiently cooled, drain the chamber in accordance with the following procedure. A r CAUTION Do not unload the exhaust bottle or drain the working chamber when the chamber is under pressure. Boiling water or steam may gush out, and you may get burned.
3. Body Service , aIMPORTANT Do not use benzine or thinner to clean the body. Also make sure that the volatile substances such as insecticides do not come into contact with the body as these substances may deteriorate [he body or strip its paint. @ Gently wipe stains from the body with a soft cloth. To remove stubborn stains, wring a cloth moistened with neutral detergent diluted with water, and wipe off the stains with it. Wipe off any moisture with a dry cloth. 5.
(2) Push in the gasket unit it hits against the metal s u r f a c e . W h e n the gasket is completely pushed in, the flat portion is positioned at a level slightly lower than the rim of the working chamber. (3) In addition, run your finger down the flat portion of the gasket to eliminate any irregularities on the gasket surface. An uneven gasket surface makes the lid difficult to close. @- (2) Flat porton d......................... ........................
Chapter 5. Specification Model Outside dimensions Inside dimensions Rated power supply 1 HV-25 HV-50 480W x 950H x 460D mm 540W x 1040H x 530D ~ n m 240 mm dia. x 550 rnm depth (Effective: 25 liters) 300 ~nrndia.
Model I I Outside dimensions I Inside dimensions Rated power supply Temperature and humidity conditions AC V f10% Single-phase 50160 Hz (more than A) 5-35"C, 10-85%RH (No condensing) 4.OKW ( ,A) 3.
Piping Diagram Pressure gauge Pressure sensor Safety valve Working chamber Exhaust valve 41 I I - Drain valve fl Exhaust bottle
Wiring Diagram U DP CP SW ELB m F B M C2 SL D C1 H Disphy printed circuit board Control printed circuit board Operation switch Power switch Transformer Fuse Backup battery Motor Capacitor for motor Solenoid P i e Eleclrolylic capacitor Heater U L S1 PS EGO LSWl LSW2 LSW3 LSW4 SSR 1X 2X 3X 4X Temperalure sensor for cmtrol Pressure sensor Water empty heating preventive device Limit switch (lever opedclose) Lima switch (lever lock) Llmit switch (exhaust vake full close) Limit swlch (bottle housing) S
Chapter 6. In the Event of Suspected Failure 1. Error Detection (Alarming) Operation Should any malfunction occur in your autoclave, the incorporated error detection circuit is triggered to assure your safety. Once the circuit is activated, the error number appears on the digital display, and further the electronic alarm sounds, indicating the occurrence of an abnormal condition. To stop the alarm sound, press the STARTISTOP switch. If an alarm is given, check the error numberhnd turn off the switch.
2 . Early Trouble .Shooting Remedy Symptom Cause Display remains off after turning on power. Check the plug and outlet first. (1) The plug is not properly inserted or is insufficiently tightened. (2) Disconnection in the power cord. (3) Defect in DISPLAY. (1) Properly insert the plug. Re t~ghren any loose parts. (2)(3) Contact us or the authorized sales outlet from which you purchased the product. No air exhausted from working chamber.
Temperature-Pressure Conversion Table for Saturated Steam in Autoclave The following data are approximate values for practical use in all autoclaves: Pressure Gauge Reading Temperature (OC) (OF) kglcm lblinch
SPARE PARTS FOR HV- AND HVE-SERIES AUTOCLAVES Catalog No. - Description ATA-25LG Lid gasket for HVIHVE-25 ATA-50LG Lid gasket for HVIHVE-50 ATA-85LG Lid gasket for HV-851110 ATA-25H Heater for HVIHVE-25, 1.
Looking for more information? Visit us on the web at http://www.artisan-scientific.com for more information: • Price Quotations • Drivers· Technical Specifications.
