Operator`s manual

44
LightCycler® 480 Gene Scanning Software
Supplementary Functions
Result Control
Result Control 2
The LightCycler® 480 Gene Scanning Software uses a control routine to check whether the
analysis has passed or failed. The result control check is only applied to a run that contains
internal melting standards; it is not applied to runs in Auto Group or Comn/Vars analysis
mode.
Negative Controls ►
If every Negative Control is designated negative, the run passes this control check. ►
If any Negative Control is not designated negative, the run fails this control check. ►
If any Negative Controls fail, the software will report no results and will inform the ►
user that the Negative Control check has failed. The error dialogue also indicates
which controls have failed this check.
Melting Standards ►
If every internal standard is designated positive, the run passes this control check. ►
If all internal standards with the same group name are put into the same group, ►
and standards with distinct group names are placed into distinct groups, the run
passes this control check.
If any two internal standards with the same group name are not grouped together, ►
the run fails this control check.
If any two internal standards with distinct group names are grouped together, the ►
run fails this control check.
If any internal standard is designated negative the run fails this control check. ►