Operator`s manual

39
Performing Gene Scanning Analysis
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Difference Plot ►
Click the Difference Plot tab in the charts area to view
the ► Normalized and Shifted Melting Curves and
the ► Normalized and Temperature Shifted Difference Plot:
The Difference Plot is determined as follows:
First the software selects the group that contains the most samples. ►
Then the standard of that group (the Base Curve) is designated the reference ►
genotype. The difference between the reference and each remaining curve is plot-
ted against Temperature.
By checking the Show Standards box, you can also display the curves of the melting
standards in the chart. These appear as black lines.
In Auto Group analysis mode, the median curve of each variant/genotype group
is defined as the standard for that group.
Click Select base curve to manually define the reference curve for the Difference Plot.
By default, the standard of the largest variant/genotype is used as base curve.
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