Operator`s manual
38
LightCycler® 480 Gene Scanning Software
Performing Gene Scanning Analysis
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In the Results table, deselect any samples you do not want included in analysis of the
results.
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Now click the Calculate button to analyze the results and determine the grouping.
The software determines variant (or genotype) groups and assigns a color and name
to each group. Simultaneously, the software calculates the Normalized and Tempera-
ture Shifted Difference Plot.
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Results of a Gene Scanning analysis include the Sample Selector with Legend Property
Selector and Legend Property buttons and the Results table. Use the Legend Property
Selector to display colors by result, by sample types, by sample preferences or by
replicate groups. Use the colored Legend Property buttons to select the display of
samples with certain properties in the MWP image, the Results table and in the
charts.
If you choose Scanning results in the Legend Property Selector, the Results table of a
Gene Scanning analysis displays the following results. The Legend Property buttons
enable you to select samples depending on the variant group:
Either 6 genotype groups (if you selected the Auto Group analysis mode) or 2 ►
genotype groups (if you selected the Comn/Vars analysis mode).
Negative and Unknown groups. ►
Checkboxes will be shown even if the corresponding groups are not found in the
data.
Group: Name of the genotype group to which this sample is assigned. ►
Variant/genotype groups that are automatically assigned by the software are
given sequential numbers (1, 2, etc.), while groups based on in-run melting stan-
dards are given names that correspond to the appropriate standards (assigned in
the Sample Editor).
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