Operator`s manual
34
LightCycler® 480 Gene Scanning Software
Performing Gene Scanning Analysis
To perform a Gene Scanning analysis:
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Using the Standards multi-select button, select the analysis mode (grouping method)
you want to apply:
Auto Group
Applies automated grouping in the absence of in-run melting
standard samples.
If, in the Sample Editor, you have not defined any sample
as a melting standard, the program will select this option by
default after it creates the analysis.
If the Auto Group analysis mode is selected, the software will as-
sign all samples to a well-defined group (up to 6 variant groups
can be assigned on the basis of melting curve shape); samples that
cannot be assigned to such a group will be placed in either the
Unknown or the Negative group.
Groups automatically assigned by the software are named
with sequential numbers (1, 2, etc.). Some groups may con-
tain no samples.
Comn/Vars
Applies automated grouping in the absence of in-run melting
standard samples.
If the Common/Variants analysis mode is selected, the software will
place all samples into either group 1 (the largest group of samples
with similar melting curve shapes, the so-called Common group),
the Variants group, the Unknown group or the Negative group.
No further grouping will be done.
Standards
(In Run)
Applies grouping based on melting standard samples included in
the run.
If, in the Sample Editor, you define samples as melting stan-
dards, the program will select this option by default after it
creates the analysis.
Groups based on internal standards are given names that
correspond to the appropriate standards (assigned in the
Sample Editor).
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Deselecting Negatives ►
If you wish, click the Negatives tab to designate samples as known negatives and
remove them from the analysis (i.e., deselect them).
Although LightCycler
®
480 Gene Scanning Software uses a negative filter to
automatically deselect negative samples, it is still possible that all negative
samples will not be detected. You will have another opportunity to correct this at
the end of the run. You can do this by using the New Call option to assign known
negative samples to the Negatives group (see below).
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