Operator`s manual

32
LightCycler® 480 Gene Scanning Software
Performing Gene Scanning Analysis
�
Click Sample Editor in the Module bar, then select the workflow Scanning. After the
workflow is selected, the LightCycler 480 Software automatically reconfigures the
Sample Editor.
�
Define the properties of the samples.
For detailed information on the Sample Editor see the LightCycler
®
480 Instrument
Operator’s Manual, section “Entering Sample Information”.
The software uses the following parameters for calculation:
Column Name Description Valid Values
Target Name
Name of the (PCR) target
(e.g., the name of the gene
amplified and detected)
Alphanumeric value
(≤ 25 characters);
default value is
“Target xxx-yyy”,
where xxx and yyy
are the excitation and
emission wavelengths
Sample Type
Type of sample
If you want to use the
“In-run Standards”
grouping option, you need
to include a Melting Stan-
dard for any genotype you
want to detect.
Choose from dropdown list:
Unknown ►
Negative Control ►
Melting Standard ►
Genotype
Genotype of melting standard
or positive control samples
This field is active only
when the Sample Type is
“Melting Standard”.
Alphanumeric value
(≤ 25 characters)
�
Click Analysis in the Module bar. In the Create New Analysis list, select Gene Scanning.
► ► ►