Operator`s manual

ManualsBrandsBosch ManualsComputer equipment480 Series

Table of Contents

Prologue 5

I Revision History ........................................................................................................................5

II Contact Addresses ...................................................................................................................5

III Trademarks .................................................................................................................................6

IV Intended Use ..............................................................................................................................6

V License Statements for the LightCycler

480 Gene Scanning Software ....................6

VI Software License Agreement .................................................................................................7

1 Program License Agreement ............................................................................................................................7

2 Grant of Software License .................................................................................................................................7

3 Limited Warranty ...................................................................................................................................................8

4 Disclaimer of Warranties ..................................................................................................................................9

5 Limitations of Remedies .....................................................................................................................................9

6 General Information ..........................................................................................................................................10

7 Intellectual Property Rights ............................................................................................................................10

8 Duration and Termination ...............................................................................................................................11

9 Import, Export and Use of the Software .................................................................................................... 11

10 Miscellaneous ..................................................................................................................................................... 11

11 Governing Law and Place of Jurisdiction ................................................................................................. 11

VII Conventions Used in this Manual ...................................................................................... 12

VIII Warnings and Precautions ................................................................................................... 13

A Gene Scanning with the LightCycler

480 System 14

1 High Resolution Melting ............................................................................................................................ 15

2 Components of the LightCycler

480 System Gene Scanning Assay .............................. 17

2.1 LightCycler

480 High Resolution Melting Dye ..................................................................................... 17

2.2 Data Analysis using the LightCycler

480 Gene Scanning Software ............................................ 18

2.3 LightCycler

480 Instrument .........................................................................................................................20

3 Advantages of the High Resolution Melting Technique ........................................................... 21

B Preparing a LightCycler

480 System Gene Scanning Experiment 22

1 Designing the Gene Scanning Assay ..................................................................................................22

2 Sample Material ............................................................................................................................................. 23

3 PCR Primers ...................................................................................................................................................... 24

4 PCR Reagents .................................................................................................................................................. 25

5 PCR Parameters ..............................................................................................................................................26

6 Rapid Detection of Ampliﬁcation Artifacts ...................................................................................... 28

C Installing the LightCycler

480 Gene Scanning Software 29

D Performing Gene Scanning Analysis 31