Operator`s manual

20
LightCycler® 480 Gene Scanning Software
Gene Scanning with the LightCycler® 480 System
Components of the LightCycler® 480 System Gene Scanning Assay
The following examples demonstrate gene scanning:
For the single-nucleotide polymorphism (SNP) G ► →T in the LPLH3 gene (163 bp
amplicon), resulting in 3 main variant groups.
For sequence variations in the MBL2 gene (219 bp amplicon), resulting in 4 main vari- ►
ant groups corresponding to the 4 most frequent haplotypes described for this gene in
literature and 3 samples of a further genetic variant.
LightCycler2.3
®
480 Instrument
High Resolution Melting analysis of nucleic acid requires that the analyzing instrument
is able to detect even small changes in fluorescence as a function of temperature-induced
DNA melting. In the LightCycler® 480 Instrument, images of DNA melting (i.e., fluores-
cence) are captured by a sensitive CCD camera and magnified to reveal subtle details in
DNA melting profiles. These profiles are then compared from sample to sample to deter-
mine relationships between the samples.
The optical components of the LightCycler® 480 System work together with its accurate
thermal control system to generate highly reproducible results from sample to sample.
Since the samples are held in a multiwell plate, the LightCycler® 480 System can analyze
many samples in a single run.