User guide

Adding regions
Regions represent the fragments in the plasmid. You can add as many regions to you map as you want or none.
To add regions:
1. Right-click the plasmid map and select Regions in the context menu.
2. In the Regions dialog box, enter in the Start and End text boxes the base pair numbers for a fragment. For exam-
ple, if a fragment includes all base pairs from 1 to 1000, type 1 and 1000 in the text boxes.
3. For the Start and End base pair numbers for the fragment to appear in the drawing, select Add Markers At
Region Ends.
4. Click Add.
5. To add more regions, repeat steps 2-4.
6. Click OK.
Adding markers
Markers let you annotate base pairs in the plasmid map. For example, to label a base pair or fragment, add a marker to
it.
To add a marker:
1. Right-click the plasmid map and select Markers in the context menu.
2. In the Markers dialog box, enter in the Position text box the number for the base pair to which you want to add a
marker.
3. In the Label text box, enter the text to identify the marker.
4. Click Add.
5. Click OK.
Moving markers
To move a marker label, click and drag the label. You can also edit the value in the label.
Resizing regions
You can resize region arrows to indicate different base pairs or extend them outward to emphasize them.
1. Hover over the end of the region’s arrow using a selection tool. The arrow’s resize handles appear and the cursor
changes to a double-arrow ( ).
2. Click and drag a resize handle to adjust the size of the arrow.
ChemBioDraw 13.0
Chapter 4: BioDraw 58 of 401