UNO™ Q&S R Continuous Bed Ion Exchange Replacement Column Instruction Manual Catalog Numbers 720-0011, 720-0013, 720-0015, 720-0031, 720-0033 and 720-0035 For Technical Service Call Your Local Bio-Rad Office or in the U.S.
Introduction UNO prepacked ion-exchange columns are designed to meet the needs of the bio-chromatographer for rapid and reproducible high-resolution separations of biomolecules including proteins, peptides and polynucleotides. The UNO Replacement columns provide an economical alternative to purchasing a new complete UNO column. The UNO Replacement column hardware utilizes the existing hardware from your worn-out UNO column.
Tubing, .020 I.D. x 15 cm (1) Cap (11) Tube end fitting, 1/4 x 28 (2) End cap (3) Shipping tube (12) Shield (4) Bed support (5) O-ring (6) Spacer (7) Distribution screen (8) Frit (9) O-ring (13) Plug (14) Centering ring (15) Glass tube (10) Glass column (16) Frit (9) Distribution screen (8) Spacer (7) O-ring (6) Bed support (5) Centering ring (15) End cap (3) Plug (14) O-ring (13) Tube end fitting, 1/4 x 28 (2) Tubing, .020 I.D. x 15 cm (1) UNO Column Cap (11) UNO Replacement Column Fig. 1.
Table 1. UNO Q Column Characteristics Column volume (ml) Recommended max. protein loading (mg) Recommended flow-rates (ml/min) Column Dimensions (mm) Maximum operating pressure (psi/MPa/bar) Q-1 1.3 20 0.5 to 5.0 7 x 35 700/4.5/48 Q-6 Q-12 6 12 80 160 0.5 to 8.0 0.5 to 10.0 12 x 53 15 x 68 700/4.5/48 700/4.5/48 Table 2. UNO S Column Characteristics Column volume (ml) Recommended max.
Product Information Catalog Number Product Description 720-0001 UNO Q-1 Column 720-0003 UNO Q-6 Column 720-0005 UNO Q-12 Column 720-0009 UNO Q Polishing Column 720-0011 UNO Q-1 R Column, replacement column 720-0013 UNO Q-6 R Column, replacement column 720-0015 UNO Q-12 R Column, replacement column 720-0021 UNO S-1 Column 720-0023 UNO S-6 Column 720-0025 UNO S-12 Column 720-0029 UNO S Polishing Column 720-0031 UNO S-1 R Column, replacement column 720-0033 UNO S-6 R Column, replacem
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