User Manual

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Universal Probes Supermix Instruction Manual
Sso7d Fusion Enzyme Technology
Bio-Rad introduced our next generation of real-time PCR supermixes using our patented
Sso7d fusion protein technology, delivering a reagent that provides effective performance
in a wide range of qPCR applications. The dsDNA-binding protein, Sso7d, stabilizes the
polymerase-template complex, increases processivity, and provides greater speed and
reduced reaction times compared to conventional DNA polymerases, without affecting PCR
sensitivity, efficiency, or reproducibility.
Key Features and Benefits

Fast qPCR results and high performance — the Sso7d fusion polymerase and optimized
buffer deliver fast reaction times via instant antibody hot-start polymerase activation and
rapid polymerization kinetics to generate exceptional qPCR results in less than 30 min

Minimal inhibition of PCR — the polymerase’s increased resistance to PCR inhibitors
ensures maximum efficiency, sensitivity, and reproducibility

Single copy detection — data illustrate high sensitivity with amplification and detection from
a single copy of target gene

Robust discrimination and reproducibility — efficient discrimination and reliable
quantification can be obtained from 1.33-fold serial dilutions of input template

GC-rich targets — ability to amplify targets where other Taq-based supermixes
may be challenged