Manual

4.1 Setup
When the SmartSpec 3000 is not carrying out an assay, you can enter the Setup routine
by pressing Setup. To proceed through Setup without changing the current setting, press
Enter. Most of the current settings can be changed by pressing Select until you see your
desired choice; sometimes you have to use the number pad to change an entry. In either case,
press Enter after the selection is made. When you have gone all the way through Setup,
SmartSpec 3000 will automatically return to Home screen. You can exit Setup at any time by
pressing Cancel. When you exit Setup, all changes will be saved.
In Setup you can specify the following:
1. Pathlength
2. Background compensation for DNA/RNA measurements.
3. Default type of nucleic acid.
4. Default method to determine DNA and RNA oligonucleotide molar extinction
coefficient and molecular weight.
5. Default number of sample replicates.
6. New operator name.
7. Current operator.
8. Serial port export.
9. Current time.
10. Current date.
11. Time display mode.
12. Date display mode.
1. Pathlength. The pathlength describes the distance that the light travels as it passes through
the sample, and is the same as the width of the cuvette. By using a cuvette with a shorter
pathlength, you can measure the absorbance of more concentrated solutions. The
relationship between absorbance, path length and sample concentration is described by
Beer’s law where
A=εbc
A is the absorbance, ε the molar extinction coefficient in liter/mole/cm, b the pathlength
in cm and c the concentration in moles/liter.
Pressing Select once will change the screen to
As you continue pressing Select, the choices change to 2 mm and then 1 mm.
7
Select Pathlength:
>=Help 10 mm
Select Pathlength:
>=Help 5 mm