Owner's manual

5.2.1. General-Purpose 2-D Rehydration/Sample

Buffer (no reducing agent)

(8 M urea, 2% CHAPS, 0.2% Bio-Lyte

3/10 ampholyte,

0.002% bromophenol blue)

Component Final Concentration Amount to Make 2 ml

Urea (FW 60.06) 8 M 0.96 g

CHAPS 2% (w/v) 0.04 g

100X Bio-Lyte 0.2% (w/v) 20 µl

3/10 ampholyte

Bromophenol Blue 0.002% (w/v) 4 µl of a 1%

(w/v) solution

Proteomic grade 1.25 ml

water

Use an ampholyte buffer that corresponds to the pH range of the IEF

separation to be performed. For example, ReadyStrip

™

micro-range buffers

with ReadyStrip micro-range IPG strips and ReadyStrip 7-10 buffer with

ReadyStrip pH 7-10 IPG strips. Bio-Lyte 3/10 ampholyte can be used with

all other ReadyStrip IPG strip pH ranges.

5.2.2. Strongly Chaotropic 2-D Rehydration/

Sample Buffers for Highly Hydrophobic Proteins

5.2.2.1. Standard Strongly Chaotropic Buffer

(no reducing agent)

(7 M urea, 2 M thiourea, 4% CHAPS, 0.2% Bio-Lyte 3/10

ampholyte, 0.002% bromophenol blue)

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