Manual

Component Final Concentration Amount to Make 2 ml
DTT
*
(FW 154.3) 50 mM 15.4 mg
100X Bio-Lyte 0.2% (w/v) 20 µl
3/10 ampholyte
**
Bromophenol Blue 0.002% (w/v) 4 µl of a 1%
(w/v) solution
*
If TBP or TCEP is substituted for DTT as the reducing agent, use at a
concentration of 2 mM.
**
Use an ampholyte buffer that corresponds to the pH range of the IEF
separation to be performed. For example, ReadyStrip
™
micro-range buffers
with ReadyStrip micro-range IPG strips and ReadyStrip 7-10 buffer with
ReadyStrip pH 7-10 IPG strips. Bio-Lyte 3/10 ampholyte can be used with
all other ReadyStrip IPG strip pH ranges.
11
4110149A5.qxd 1/15/2003 2:49 PM Page 14