Owner manual

Items Required But Not Provided

• 2.0 ml microcentrifuge tubes (for example, VWR catalog

#20170-237).

• Disposable round-bottom tubes for plant sample

extraction (for example, VWR catalog # 60818-725).

• ReadyPrep 2-D cleanup kit (Bio-Rad catalog #163-2130)

for plant sample extraction. When working with plant leaf

tissue, removal of interfering compounds, such as

phenolics, is essential for high quality 2-D gels with

minimal horizontal streaking.

• 50 ml conical centrifuge tube(s), disposable, (for

example, VWR catalog #20171-030).

• 2 clean 125–150 ml beakers or bottles plus stirring bars.

• Ultracentrifuge, rotor and tubes capable of spinning at

100,000 x g. Typically, tubes should have ~30 ml

capacity.

• Microcentrifuge capable of spinning at 12–16,000 x g

at 4°C.

• Sonicator

• Benzonase (endonuclease) (for example, Sigma

catalog #E1014 or #E8263)

• 1 M MgCl

solution

• Protease inhibitor(s) (optional)

• Carrier ampholytes (for example, Bio-Lyte



3/10

ampholyte, Bio-Rad catalog #163-2094)

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