Instruction Manual

Instruction Manual | 54 | Automated Droplet Generator4 | Automated Droplet Generator
QX200 AutoDG Droplet Digital PCR System
Table 2. Additional materials required for Droplet Digital PCR.
Component Description Catalog #
Automated Droplet Generator
DG32 Cartridges Microfluidic cartridge with gasket used to mix sample and 186-4108, 186-4109
oil to generate droplets in the Automated Droplet Generator
Droplet Generation Oils Automated Droplet Generation Oil for EvaGreen 186-4112
Automated Droplet Generation Oil for Probes 186-4110
PCR Supermixes QX200
™
ddPCR
™
EvaGreen
®
Supermix 186-4033, 186-4034, 186-4035,
186-4036
ddPCR
™
Supermix for Probes (no dUTP) 186-3023, 186-3024, 186-3025
ddPCR
™
Supermix for Probes 186-3026, 186-3010, 186-3027,
186-3028
One-Step RT-ddPCR Kit for Probes 186-3021, 186-3022
Controls QX200 Buffer Control Kit for EvaGreen 186-4052
QX200 Buffer Control Kit for Probes 186-3052
Pipet Tips Pipet Tips for Automated Droplet Generator 186-4120, 186-4121
Pipet Tip Bin Pipet Tip Waste Bins for AutoDG 186-4125
96-well PCR Plates twin.tec 96-well semi-skirted plates Eppendorf 951020362
Sealer and Thermal Cycler
Foil Seal Pierceable Foil Heat Seal 181-4040
Plate Sealer PX1
™
PCR Plate Sealer 181-4000
Thermal Cycler C1000 Touch
™
Thermal Cycler with 96-deep well 185-1197
reaction module
QX200 Droplet Reader*
Droplet Reader Oil ddPCR Droplet Reader Oil 186-3004
* For information on components shipped with QX200 Droplet Reader, see part number 10031906.
1.3 Installation and General Operation
Automated Droplet Digital PCR involves the following steps (4.5–5 hours for the complete workflow):
1. Prepare PCR-ready samples — combine nucleic acid sample (DNA or RNA), primers, and probes (FAM,
VIC, or HEX) or intercalating dye (EvaGreen) with Bio-Rad ddPCR supermix (see Table 2).
2. Make droplets — load 22 μl of the ddPCR reaction into a 96-well PCR plate, then load the plate and required
consumables into the Automated Droplet Generator to partition the sample into droplets. The Automated
Droplet Generator uses microfluidics to combine oil and aqueous sample to generate the nanoliter-sized
droplets required for ddPCR analysis. It processes up to 96 samples at a time in less than 45 min.