User Manual

Preface
QX100 Droplet Reader and QuantaSoft Software Instruction Manual QX100 Droplet Reader and QuantaSoft Software Instruction Manual | iii
Table of Contents
Chapter 1 QX100
™
Droplet Digital
™
PCR System 1
1.1 Introduction 1
1.2 System Components 2
1.3 Droplet Digital PCR Workflow 2
1.4 System Setup and General Operation Instructions 4
Chapter 2 Using the QX100
™
Droplet Reader 5
Chapter 3 Using QuantaSoft
™
Software 9
3.1 Setup 10
3.1.1 Using the Well Editor 11
3.1.2 Using the Experiment Editor 12
3.1.3 Using the Advanced Options 13
3.2 Run 13
3.3 Analyze 14
3.3.1 Viewing Channel Data (1D Amplitude) 15
3.3.2 Viewing Clustering Plots (2D Amplitude) 17
3.3.3 Viewing Concentration Data (Concentration) 18
3.3.4 Viewing Copy Number Data (Copy Number) 18
3.3.5 Viewing Ratio Data (Ratio) 19
3.3.6 Viewing Events 19
Chapter 4 Specifications and Maintenance 21
4.1 Specifications 21
4.2 Maintenance 22
4.2.1 General Maintenance Procedures 22
4.2.2 Replacing Droplet Reader Oil and Removing Waste 22
Appendix A Ordering Information 23