User Manual

Problem Possible Cause Recommended Solution

RNA is Cells grown in either For cells grown in monolayer,

degraded monolayer or aspirate the growth medium

(continued) suspension were and then add PureZOL

washed prior to directly to the plate. No

homogenization with washing or trypsinization is

PureZOL necessary. For cells grown in

suspension, pellet the cells

and aspirate growth

medium, then add PureZOL

directly to the pellet

Starting tissue sample Make sure that starting

was not immediately material is immediately

frozen, or had gone processed following

through several dissection. Alternatively, the

freeze-thaw cycles starting material can be

before RNA immediately frozen after

purification was dissection. Once frozen, do

performed not subject starting material

to freeze-thaw cycles.

Cultured cells were Cells should be lysed directly

dispersed by trypsin in PureZOL RNA isolation

reagent. Do not wash cells

or trypsinize prior to lysing

Genomic DNA Some of the white Leave some of the aqueous

contamination interphase (after phase phase solution behind to

separation) was avoid transferring the white

transferred with the interphase with the aqueous

aqueous phase phase (see step 6 in the

protocol)

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