LIT84B 9/1/98 8:36 AM Page FC1 Prefilled Poly-Prep® Columns for Ion Exchange Chromatography Instruction Manual
LIT84B 9/1/98 8:36 AM Page FC2 Table of Contents Section 1 Introduction ............................1 Section 2 Column Description................2 Section 3 Instructions For Use ...............2 Section 4 Ion Exchange Capacity ..........3 Section 5 Flow Rate Characteristics......4 Section 6 Chemical Compatibility .........5 Section 7 Temperature Limits ...............6 Section 8 Troubleshooting ......................6 Section 9 Ordering Information ............
LIT84B 9/1/98 8:37 AM Page 1 Section 1 Introduction Prefilled Poly-Prep columns are small polypropylene columns containing ion exchange resins. These columns combine the analytical quality and wide selection of Bio-Rad’s ion exchange resins with the convenience of small, disposable columns. Poly-Prep columns are especially useful in sample preparation for HPLC, GC, IC, and AA analysis.
LIT84B 9/1/98 8:37 AM Page 2 Poly-Prep columns are ideal for routine applications in which a small amount of chromatographic medium is used and then discarded, or for multiple sample clean-up applications. They are a time-saving and versatile replacement for Pasteur pipets and other improvised columns. Section 2 Column Description The graduate 0.8 x 4 cm columns, constructed of high density polypropylene, hold a standard bed volume of 2 ml of chromatographic media, and include an integral 10 ml reservoir.
LIT84B 9/1/98 8:37 AM Page 3 2. Remove the column cap to allow the eluant to flow freely. 3. Snap off the seal at the bottom of the column. 4. Monitor the level of liquid in the reservoir, and do not allow the resin to become dry. 5. Rinse the resin with several bed volumes (at least 2 ml) of the sample solvent. Discard rinse. 6. Add sample. 7. Collect effluent. 8. To reduce the flow rate or stop the flow, use a Luer 3-way stopcock or the yellow end-cap.
LIT84B 9/1/98 8:37 AM Page 4 The ion exchange capacity, usually expressed as meq/ml of resin, is equivalent to the number of ion exchange sites per unit volume of resin. The following examples shows the calculated volume of 1 N sodium hydroxide that would be required to convert the 2 ml of resin in a prefilled AG 50W-X8 (H+) PolyPrep column from the hydrogen form to the sodium form. In general, it is recommended to use only 70-80% of the theoretical capacity of an ion exchange resin.
LIT84B 9/1/98 8:37 AM Page 5 Section 6 Chemical Compatibility Table 2 gives the chemical compatibility of prefilled Poly-Prep columns. While the column is often very resistant, the compatibility of the resin must be considered. Table 2.
LIT84B 9/1/98 8:37 AM Page 6 Section 7 Temperature Limits Increases in temperature tend to decrease resin selectivity, and can be used to decrease elution times. Increased temperature also increases exchange kinetics, resulting in sharper resolution. However, some temperature limits must be observed with ion exchange resins. Table 3 gives the maximum temperature for each resin packing. Table 3.
LIT84B 9/1/98 8:37 AM Page 7 can be removed easily by rinsing with 2-3 bed volumes of mobile phase. 3. Effluent is red: After prolonged storage, sulfonic acid will sometimes leach from AG 50 strong cation exchange resin, yielding a red effluent. Wash the resin with mobile phase or water until the color is no longer visible. 4. Cloudy effluent: The initial rinse may contain a small quantity of resin. Discard the wash and continue as normal. 5. Flow rate too fast: Use a stopcock to adjust the flow rate. 6.
LIT84B 9/1/98 8:37 AM Page 8 Section 9 Ordering Information Catalog Number Column Packing Ionic Form Mesh Size 731-6211 AG 1-X8 Resin, 50 Cl– 100-200 731-6212 AG 1-X8 Resin, 50 Cl– 200-400 731-6221 AG 1-X8 Resin, 50 HCOO– 200-400 731-6247 AG 2-X8 Resin, 50 Cl– 100-200 731-6225 AG 50W-X4 Resin, 50 H+ 200-400 731-6213 AG 50W-X8 Resin, 50 H+ 100-200 731-6214 AG 50W-X8 Resin, 50 H+ 200-400 731-6217 Chelex 100 Resin, 50 Na+ 100-200 731-6232 Chelex 100 Resin, 50 Na+ 200-400
LIT84B 9/1/98 8:37 AM Page BC1 Bio-Rad Laboratories, 2000 Alfred Nobel Dr.
