Software User Manual

Analyzing Melt Data
44
Plate View Spreadsheet
Select Plate View spreadsheet to see a plate map of the data. Figure 34 shows the Plate view
spreadsheet as plate map.
Figure 34. Plate spreadsheet in Precision Melt Data tab.
The Plate View spreadsheet includes the type of information shown in Table 18, including the
selected fluorophore in the plate map.
Select the Raw RFU spreadsheet to view raw fluorescence readings for each well acquired at
each temperature step of the melt curve protocol. The well number appears at the top of each
column, and temperature appears to the left of each row (Figure 35).
Figure 35. Raw RFU spreadsheet in the Precision Melt Data tab.
Table 18. Plate spreadsheet contents
Information Description
Content A combination of the Sample Type and Replicate # loaded in
the well in the Plate Editor
Sample Sample name loaded in the well in the Plate Editor
Cluster Cluster name
Color Cluster color